Difference between revisions of "Costa 1997 Am J Physiol Cell Physiol"
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{{Publication | {{Publication | ||
|title=Costa LE, Méndez G, Boveris A (1997) Oxygen dependence of mitochondrial function measured by high-resolution respirometry in long-term hypoxic rats. Am. J. Physiol.Cell Physiol. 273: C852-C858. | |title=Costa LE, Méndez G, Boveris A (1997) Oxygen dependence of mitochondrial function measured by high-resolution respirometry in long-term hypoxic rats. Am. J. Physiol.Cell Physiol. 273: C852-C858. | ||
|authors=Costa LE, Méndez G, Boveris A | |authors=Costa LE, Méndez G, Boveris A | ||
|year=1997 | |year=1997 | ||
|journal= | |journal=Am. J. Physiol.Cell Physiol. | ||
|abstract=Respiration and oxidative phosphorylation were investigated in tightly coupled mitochondria isolated from liver and heart of rats submitted to a simulated altitude of 4,400 m for 14-15 mo and their corresponding controls at sea level. High-resolution respirometry was utilized to determine the apparent Michaelis-Menten constant for ADP and O<sub>2</sub> (K(m)-ADP and K(m)-O<sub>2</sub>, respectively), the latter under active and resting states of mitochondrial respiration. The K(m)-O<sub>2</sub> in mitochondria isolated from normoxic rats was higher for active (state 3) than for resting (state 4) respiration; the values decreased from 1.5 and 1.7 to 0.25 and 0.30 microM in heart and liver mitochondria, respectively. The K(m)-O<sub>2</sub> values found in the active state suggest a role for the normally occurring intracellular PO2 range reported in the literature in the regulation of cellular respiration. No changes were found in the ADP or O<sub>2</sub> dependence of respiration in the mitochondria isolated from long-term acclimatized rats compared with their controls, indicating that the intrinsic properties and the efficiency of mitochondria do not change as a consequence of adaptation to hypoxia. | |abstract=Respiration and oxidative phosphorylation were investigated in tightly coupled mitochondria isolated from liver and heart of rats submitted to a simulated altitude of 4,400 m for 14-15 mo and their corresponding controls at sea level. High-resolution respirometry was utilized to determine the apparent Michaelis-Menten constant for ADP and O<sub>2</sub> (K(m)-ADP and K(m)-O<sub>2</sub>, respectively), the latter under active and resting states of mitochondrial respiration. The K(m)-O<sub>2</sub> in mitochondria isolated from normoxic rats was higher for active (state 3) than for resting (state 4) respiration; the values decreased from 1.5 and 1.7 to 0.25 and 0.30 microM in heart and liver mitochondria, respectively. The K(m)-O<sub>2</sub> values found in the active state suggest a role for the normally occurring intracellular PO2 range reported in the literature in the regulation of cellular respiration. No changes were found in the ADP or O<sub>2</sub> dependence of respiration in the mitochondria isolated from long-term acclimatized rats compared with their controls, indicating that the intrinsic properties and the efficiency of mitochondria do not change as a consequence of adaptation to hypoxia. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/9316405 PMID: 9316405] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/9316405 PMID: 9316405] | ||
}} | }} | ||
Revision as of 17:10, 8 October 2010
| Costa LE, Méndez G, Boveris A (1997) Oxygen dependence of mitochondrial function measured by high-resolution respirometry in long-term hypoxic rats. Am. J. Physiol.Cell Physiol. 273: C852-C858. |
Costa LE, Méndez G, Boveris A (1997) Am. J. Physiol.Cell Physiol.
Abstract: Respiration and oxidative phosphorylation were investigated in tightly coupled mitochondria isolated from liver and heart of rats submitted to a simulated altitude of 4,400 m for 14-15 mo and their corresponding controls at sea level. High-resolution respirometry was utilized to determine the apparent Michaelis-Menten constant for ADP and O2 (K(m)-ADP and K(m)-O2, respectively), the latter under active and resting states of mitochondrial respiration. The K(m)-O2 in mitochondria isolated from normoxic rats was higher for active (state 3) than for resting (state 4) respiration; the values decreased from 1.5 and 1.7 to 0.25 and 0.30 microM in heart and liver mitochondria, respectively. The K(m)-O2 values found in the active state suggest a role for the normally occurring intracellular PO2 range reported in the literature in the regulation of cellular respiration. No changes were found in the ADP or O2 dependence of respiration in the mitochondria isolated from long-term acclimatized rats compared with their controls, indicating that the intrinsic properties and the efficiency of mitochondria do not change as a consequence of adaptation to hypoxia.
Labels:
Stress:Hypoxia Organism: Rat Tissue;cell: Cardiac Muscle"Cardiac Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property., Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
