Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Cizmarova Beata"

From Bioblast
Line 1: Line 1:
{{EAGLE
|COST= Member
|COST WG1= WG1
|COST WG4= WG4
}
{{Person
{{Person
|lastname=Velika
|lastname=Velika
Line 21: Line 26:
}}
}}
{{Labelingperson}}
{{Labelingperson}}
{{EAGLE
}
|COST= Member
|COST WG1= WG1
|COST WG4= WG4
}}





Revision as of 10:54, 6 July 2017

{{EAGLE |COST= Member |COST WG1= WG1 |COST WG4= WG4 }

Name Velika Beata, PhD, RNDr.
Institution
Beata Velika

Pavol Jozef Šafárik University in Kosice

Faculty of Medicine
Department of Medical and Clinical Biochemistry

Visiting scientist

  • Research on: Cryopreservation and respiration of blood cells

Beata Velika joined OROBOROS in January 2017 within the framework
of the Postdoc Scholarship Action Austria.

Address Trieda SNP 1, 040 11
City Košice
State/Province
Country Slovakia
Email [email protected]
Weblink
O2k-Network Lab


Labels:



Publications

 PublishedReference
MitoEAGLE blood cells 12020Åsander Frostner Eleonor*, Aburel Oana M*, Avram Vlad F*, Calabria Elisa, Castelo Rueda Maria Paulina*, Chamkha Imen*, Čižmárová Beata, Danila Maria-Daniela*, Doerrier Carolina*, Eckert Gunter P*, Ehinger Johannes K, Elmer Eskil*, Garcia-Souza Luiz F*, Gnaiger Erich*, Hoppel Florian*, Karabatsiakis Alexander*, Keppner Gloria, Kidere Dita*, Krako Jakovljević Nina, Labieniec-Watala Magdalena*, Lelcu Theia*, Micankova Petra, Michalak Slawomir*, Molina Anthony JA*, Pavlovic Kasja, Pichler Irene*, Piel Sarah, Rousar Tomas, Rybacka-Mossakowska Joanna, Schartner Melanie, Siewiera Karolina*, Silaidos Carmina*, Sjövall Fredrik*, Sobotka Ondrej*, Sumbalova Zuzana*, Swiniuch Daria, Vernerova Andrea*, Volani Chiara*, Vujacic-Mirski Ksenija*, Watala Cezary* (2020) Interlaboratory guide to mitochondrial respiratory studies with peripheral blood mononuclear cells and platelets. - Updated: 2020-03-06 - *Confirmed
BEC 2020.1 doi10.26124bec2020-0001.v12020Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
Evinova 2020 J Membr Biol2020Evinova A, Cizmarova B, Hatokova Z, Racay P (2020) High‑resolution respirometry in assessment of mitochondrial function in neuroblastoma SH‑SY5Y intact cells. J Membr Biol 253:129-36.
Sumbalova 2018 Nova Sciences2018Sumbalová Z, Garcia-Souza LF, Cizmarova B, Volani C, Gnaiger E (2018) Analysis of mitochondrial function in human blood cells. In: Recent advances in mitochondrial medicine and coenzyme Q10. Gvozdjáková A, Cornelissen G, Singh RB eds, Nova Sciences:255-68.
Gatterer 2018 J Sports Sci Med2018Gatterer H, Menz V, Salazar-Martinez E, Sumbalova Z, Garcia-Souza LF, Cizmarova B, Gnaiger E, Burtscher M (2018) Exercise performance, muscle oxygen extraction and blood cell mitochondrial respiration after repeated-sprint and sprint interval training in hypoxia: a pilot study. J Sports Sci Med 17:339-347.

Abstracts

 PublishedReference
Hatokova 2019 MiPschool Coimbra2019
MiPsociety
Influence the passage number on a respiration of SH-SY 5Y cells.
Evinova 2019 MiPschool Coimbra2019
MiPsociety
High resolution respirometry of neuroblastoma SK-N-SH and SH-SY 5Y cell lines.
Garcia-Souza 2018 Life Sciences Meeting 2018 Innsbruck AT2018A respirometric cell viability test for peripheral-blood mononuclear cells and platelets.
Evinova 2018 MiP20182018
MiPsociety
High-resolution respirometry of neuroblastoma SH-SY 5Y cell line.
Cizmarova 2018 MiP20182018
Velika Beata
High-resolution respirometry of neuroblastoma SH-SY 5Y cell line.
Garcia-Souza 2017 MiP20172017
Luiz Garcia
Short-term acclimatization to moderate altitude does not affect respiration of peripheral blood mononuclear cells and platelets.
Garcia-Souza 2017 MiPschool Obergurgl2017
Luiz Garcia-Souza
Assessment of mitochondrial respiratory function in cryopreserved platelets.
Sumbalova 2017 MiP2017 WG42017
Zuzana Sumbalova
Purity of blood cell fractions in respirometric studies: peripheral blood mononuclear cells and platelets.
Cizmarova 2017 MiPschool Obergurgl2017
Beata Cizmarova
The scope of cryopreservation in blood cells for functional mitochondrial diagnosis.
Garcia-Souza 2017 MITOEAGLE Obergurgl2017
GarciaL.JPG
Towards a database on PBMC mitochondrial respiration: first steps on a literature survey linked to the MitoFit experimental data.
Cizmarova 2017 MITOEAGLE Obergurgl2017
Beata Cizmarova
High-resolution respirometry and viability of cryopreserved blood cells.
Cizmarova 2017 Abstract MITOEAGLE Barcelona2017
COST Action MitoEAGLE
Optimization of cryopreservation of human peripheral blood mononuclear cells and platelets for high-resolution respirometry.
Cizmarova 2017 MiP20172017
Beate Cizmarova
PBMC cryopreservation and evaluation for functional mitochondrial diagnosis.
Laner 2017 Abstract EUROMIT2017 Cologne2017
EUROMIT2017
K-Regio MitoFit and MitoEAGLE – towards a global data bank on mitochondrial physiology.

}


Participated at


MITOEAGLE Short-Term Scientific Mission

Motivation letter
My name is Beáta Veliká and I work as Research specialist at the Pavol Jozef Šafárik University in Kosice, Department of Medical and Clinical Biochemistry, Faculty of Medicine, Slovakia. I graduated at the Faculty of Natural Science, at the University of Pavol Jozef Šafárik in Košice, Slovakia. The field of my study was biology and chemistry. After Master’s studies I continued in PhD study, at the Faculty of Medicine, at the University of Pavol Jozef Šafárik in Košice, Slovakia. The major field of my studies was Clinical Biochemistry. The topic of my dissertation work was antioxidant properties of natural and synthetic compounds.
During my study and work I specialized in fluorescence spectroscopy method, I have experiences with measuring of SFF, EEM, synchronous spectra of different biological fluids, and I have experiences with different isolation methods, including mitochondria isolation, and measuring of their native fluorescence and respiration by Clark electrode.
Overview of the proposed activities will be performed:
The short–term project is focused on the optimalization of protocols for cryopreservation of human cells which are used as the study model of mitochondria respiration and H2O2 production. The use of human blood cells will provide a significant stimulus for the entire field of mitochondrial medicine, because the mitochondrial function of blood cells is linked with numerous diseases and metabolic conditions in the human body, and the protocols developed during the project will lead to the standardization of the procedures, and should be used for studying physiological and pathological mechanisms in mitochondria. The research project should develop optimized conditions of cryopreservation for valid subsequent respirometric measurements of stored cells. The establishment of adequate conditions is very important, because reports by numerous groups that have previously evaluated the use of cryopreserved cells and tissue samples for later analysis by respirometry were both positive as well as negative.
We will work on the standardization of the procedures how to separate or isolate blood cells. We will test the different conditions of freezing and optimize the freezing protocols by testing appropriate media for cryopreservation (10, 20, 30% DMSO in FCS), we will also test the proper cell density at which cells should be stored (concentrated, diluted) and the time, for how long the cells can be maintained in a cryopreserved state. We will also optimize the thawing procedure of the cells, the effect of quick and slow freezing with freeze container enabling freezing with 1°C/min, and their viability and recovery after freezing. After that we will optimize protocols with defined substrate combinations, uncoupler and inhibitor titrations (SUIT - substrate-uncoupler-inhibitor titration protocol), which will be applied for the measurement of respiration in combination with fluorescence methods. We will evaluate the respiration and H2O2 production of permeabilized blood cells. We will apply high-resolution respirometry with using the Oxygraph-2k and the O2k-Fluo LED2-Module, in collaboration with experts in the OROBOROS laboratory, and we will apply fluorometry with Amplex Red reagent (AmR), to detect hydrogen peroxide (H2O2) production. The most appropriate conditions for the respirometric study of the different cell types will be determined, anticipating that cells with different functions may require different respiration media and/or substrate combinations for the adequate respirometric characterization. These measurements will be performed with the Oxygraph-2k and the O2k-Fluo LED2-Module, in collaboration with experts in the OROBOROS laboratory.
I would like to carry out the project in cooperation with the research group at the Mitochondrial Research Laboratory of Dr. Erich Gnaiger in Innsbruck, Austria. The laboratory of Dr. Erich Gnaiger contains very high standard laboratory equipment, with a number of specialists in the field of mitochondrial physiology and instrument development working in the lab. The project will be realized at the Mitochondrial Research Laboratory of Prof. Dr. Erich Gnaiger (Medical University of Innsbruck; K-Regio project MitoFit) in collaboration with the scientific company OROBOROS INSTRUMENTS Corp.
STSM request: download pdf