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Difference between revisions of "Carnitine"

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|description='''Carnitine''' is an important factor for the transport of long-chain fatty acids bound to carnitine ([[carnitine acyltransferase]]) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
|description='''Carnitine''' is an important factor for the transport of long-chain fatty acids bound to carnitine ([[carnitine acyltransferase]]) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
}}
}}
{{MitoPedia topics
__TOC__
|mitopedia topic=Substrate and metabolite
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==
::::* Car:  '''L-carnitine:''' Sigma Aldrich; C0158; FW=161.2
::::* Car:  '''L-carnitine:''' Sigma Aldrich; C0158; FW=161.2
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::::* [[Cahova_2014_Appl_Physiol_Nutr_Metab]]
::::* [[Cahova_2014_Appl_Physiol_Nutr_Metab]]
::::* [[Wessels_2015_Am_J_Physiol]]
::::* [[Wessels_2015_Am_J_Physiol]]
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
}}

Revision as of 17:18, 10 September 2020


high-resolution terminology - matching measurements at high-resolution


Carnitine

Description

Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.

Abbreviation: Car

Application in HRR

  • Car: L-carnitine: Sigma Aldrich; C0158; FW=161.2
Preparation of 250 mM stock solution (dissolved in H2O):
  1. Weigh 100 mg of Carnitine.
  2. Add 2.48 mL H2O.
  3. Divide into 100 µL portions.
  4. Store frozen at -20°C.
O2k Manual titration
  • Titration volume: 4 µL 250 mM stock using a 10 µL Hamilton syringe (2 mL O2k-Chamber).
  • Final concentration: 0.5 mM.


Feeding carnitine


MitoPedia topics: Substrate and metabolite