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Difference between revisions of "Carboxyatractyloside"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=Cat
|abbr=Cat
|description='''Carboxyatractyloside''' is a highly selective and potent inhibitor of the [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]]. Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic side of the inner membrane and blocke the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the ''c'' conformation of  ANT leading to permeability transition pore (PTP) opening, loss of  mitochondrial membrane potential, and apoptosis.        
|description='''Carboxyatractyloside''' is a highly selective and potent inhibitor of the [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]]. Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic side of the inner membrane and blocke the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the ''c'' conformation of  ANT leading to permeability transition pore (PTP) opening, loss of  mitochondrial membrane potential, and apoptosis.
|info=[[MiPNet03.02]]
|info=[[MiPNet03.02]]
|type=Inhibitor PS
|type=Inhibitor PS
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|type=Inhibitor PS
|type=Inhibitor PS
}}
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==


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'''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>0):  
'''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>0):  


::1) weigh 4.02 mg of Carboxyatractyloside  
::1) weigh 4.7 mg of Carboxyatractyloside  
::2) dissolve in 1 ml H<sub>2</sub>O   
::2) dissolve in 1 ml H<sub>2</sub>O   
::3) divide into 0.2 ml aliquots  
::3) divide into 0.2 ml aliquots  

Revision as of 14:15, 15 January 2014


high-resolution terminology - matching measurements at high-resolution


Carboxyatractyloside

Description

Carboxyatractyloside is a highly selective and potent inhibitor of the Adenine nucleotide translocator (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic side of the inner membrane and blocke the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.

Abbreviation: Cat

Reference: MiPNet03.02



MitoPedia topics: Inhibitor 

Application in HRR

Cat: Carboxyatractyloside (Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H44O18S2*Kx); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); Alternative source: Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H43O18S2K3*3H2O), 5 mg, store at -20 °C, FW = 939.1)


Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Very toxic!


Preparation of 5 mM stock solution (dissolved in H20):

1) weigh 4.7 mg of Carboxyatractyloside
2) dissolve in 1 ml H2O
3) divide into 0.2 ml aliquots
4) store frozen at -20 °C


Comment: Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.


* Talk:Carboxyatractyloside