Difference between revisions of "Carboxyatractyloside"

Revision as of 08:53, 18 May 2012

high-resolution terminology - matching measurements at high-resolution

Carboxyatractyloside

Description

Carboxyatractyloside is a highly selective and potent inhibitor of the Adenine nucleotide translocator (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic side of the inner membrane and blocke the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.

Abbreviation: Cat

Reference: MiPNet03.02

MitoPedia topics: Inhibitor

Application in HRR

Cat: Carboxyatractyloside (Carboxyatractyloside potassium salt from Xanthium sibiricum, C₃₁H₄₄O₁₈S₂*K_x); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); Alternative source: Carboxyatractyloside potassium salt from Xanthium sibiricum (C₃₁H₄₃O₁₈S₂K₃*3H₂O) Merck / Calbiochem 216291-5MG, 5 mg, store at -20 °C, FW = 939.1

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Very toxic!

Preparation of 5 mM stock solution (dissolved in H₂0):

1) weigh 4.5 mg of Carboxyatractyloside

2) dissolve in 1 ml H₂O

3) divide into 0.2 ml aliquots

4) store frozen at -20 °C

Comment: Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.

* Talk:Carboxyatractyloside

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Cat
-|description='''Carboxyatractyloside''' is an inhibitor of the [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]]. It is a highly selective inhibitor of cytosolic side-specific mitochondrial ADP/ATP carrier; i.e. adenine nucleotide  translocase (ANT); causes stabilization of the ''c'' conformation of  ANT leading to permeability transition pore (PTP) opening, loss of  mitochondrial membrane potential, and apoptosis.
+|description='''Carboxyatractyloside''' is a highly selective and potent inhibitor of the [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]]. Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic side of the inner membrane and blocke the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the ''c'' conformation of  ANT leading to permeability transition pore (PTP) opening, loss of  mitochondrial membrane potential, and apoptosis.
 |info=[[MiPNet03.02]]
 |type=Inhibitor PS
@@ Line 25: / Line 25: @@
 ::4) store frozen at -20 °C
-'''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
-::* Titration volume: 30 µl using a 50 µl syringe
-::* Final conc. in 2 ml O2k-chamber: 0.75 mM
 '''Comment:''' Cat is considered to be much more specific than [[atractyloside|Atractyloside]] (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.
-Cat is available from Sigma (C4992; only 2 mg available), Calbiochem (216201-2MG or 216201-5MG).
   * [[Talk:Carboxyatractyloside]]