Difference between revisions of "Carboxyatractyloside"
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|description='''Carboxyatractyloside''', Cat, is a highly selective and potent inhibitor of the [[adenine nucleotide translocator]] (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic (positive) side of the inner membrane and blocks the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the ''c'' conformation ofĀ ANT leading to permeability transition pore (PTP) opening, loss ofĀ mitochondrial membrane potential, and apoptosis. | |description='''Carboxyatractyloside''', Cat, is a highly selective and potent inhibitor of the [[adenine nucleotide translocator]] (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic (positive) side of the inner membrane and blocks the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the ''c'' conformation ofĀ ANT leading to permeability transition pore (PTP) opening, loss ofĀ mitochondrial membrane potential, and apoptosis. | ||
|info=[[MiPNet03.02]] | |info=[[MiPNet03.02]] | ||
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{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
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__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | |||
|abbr=Cat | |||
|trivial name=Carboxyatractyloside | |||
|complete name=carboxyatractyloside potassium salt from ''Xanthium sibiricum'' | |||
|chem formula=C<sub>31</sub>H<sub>46</sub>O<sub>18</sub>S<sub>2</sub>*xK<sup>+</sup> | |||
|molar mass=802.99 | |||
|vendor=Sigma-Aldrich | |||
|product number=C4992 | |||
|store at=-20 Ā°C | |||
|sensitivity= | |||
|cas=77228-71-8 | |||
|h statements=H301, H311, H331 | |||
|h info=toxic if swallowed, toxic in contact with skin, toxic if inhaled | |||
::: '''Cat:Ā Carboxyatractyloside'''Ā (carboxyatractyloside potassium salt from ''Xanthium sibiricum'',Ā C<sub>31</sub>H<sub>44</sub>O<sub>18</sub>S<sub>2</sub>*K<sub>x</sub>);Ā Sigma C 4992,Ā 2 mg, store at -20 Ā°C; M = 802.99 gĀ·mol<sup>-1</sup> (free acid basis); | }}<!--::: '''Cat:Ā Carboxyatractyloside'''Ā (carboxyatractyloside potassium salt from ''Xanthium sibiricum'',Ā C<sub>31</sub>H<sub>44</sub>O<sub>18</sub>S<sub>2</sub>*K<sub>x</sub>);Ā Sigma C 4992,Ā 2 mg, store at -20 Ā°C; M = 802.99 gĀ·mol<sup>-1</sup> (free acid basis);--> | ||
:::: Alternative source: Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from ''Xanthium sibiricum'', C<sub>31</sub>H<sub>43</sub>O<sub>18</sub>S<sub>2</sub>K<sub>3</sub>*3H<sub>2</sub>O), 5 mg, store at -20 Ā°C, M = 939.1 gĀ·mol<sup>-1</sup>. | |||
:::: <spanĀ style="color:#8B008B"> '''Caution:''' Chemicals stored inĀ the fridgeĀ or freezer should be allowed to reach room temperatureĀ beforeĀ opening. '''Very toxic'''!</span> | <!--:::: <spanĀ style="color:#8B008B"> '''Caution:''' Chemicals stored inĀ the fridgeĀ or freezer should be allowed to reach room temperatureĀ beforeĀ opening. '''Very toxic'''!</span>--> | ||
<!-- | <!-- | ||
:::: '''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>O): Ā | :::: '''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>O): Ā | ||
Revision as of 11:38, 10 June 2021
- high-resolution terminology - matching measurements at high-resolution
Carboxyatractyloside
Description
Carboxyatractyloside, Cat, is a highly selective and potent inhibitor of the adenine nucleotide translocator (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic (positive) side of the inner membrane and blocks the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.
Abbreviation: Cat
Reference: MiPNet03.02
MitoPedia topics:
Inhibitor
Application in HRR
- Cat: Carboxyatractyloside (carboxyatractyloside potassium salt from Xanthium sibiricum; C31H46O18S2*xK+), Sigma-Aldrich: C4992, store at -20 Ā°C, CAS: 77228-71-8, M = 802.99 gĀ·mol-1
- Hazard statements: H301, H311, H331; toxic if swallowed, toxic in contact with skin, toxic if inhaled
- Cat: Carboxyatractyloside (carboxyatractyloside potassium salt from Xanthium sibiricum; C31H46O18S2*xK+), Sigma-Aldrich: C4992, store at -20 Ā°C, CAS: 77228-71-8, M = 802.99 gĀ·mol-1
- Alternative source: Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H43O18S2K3*3H2O), 5 mg, store at -20 Ā°C, M = 939.1 gĀ·mol-1.
- Preparation of 2 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- For a vial with 2 mg of carboxyatractyloside: dissolve in 1065 ĀµL H2O.
- Divide into 100 ĀµL aliquots.
- Store at -20 Ā°C.
- Ā» O2k manual titrations Ā» MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1-5 ĀµL using a 10 ĀµL Hamilton syringe.
- Final concentration: 1-5 ĀµM.
- Preparation of 0.5 mM solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 50 ĀµL of the 2 mM stock solution.
- Add 150 ĀµL H2O.
- Ā» O2k manual titrations Ā» MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1-5 ĀµL using a 10 ĀµL Hamilton syringe.
- Final concentration: 1-5 ĀµM.
- Comment: Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (ĀµM). With Atr, a very high concentration (mM) is required for full inhibition. Cat appears to be little permeable to intact cells and can thus only be applied in permeabilized cells and tissues or in isolated mitochondria (Ruas 2016 PLOS_ONE).
Ā» Talk:Carboxyatractyloside
