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Difference between revisions of "Carboxyatractyloside"

From Bioblast
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|description='''Carboxyatractyloside''', Cat, is a highly selective and potent inhibitor of the [[adenine nucleotide translocator]] (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic (positive) side of the inner membrane and blocks the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the ''c'' conformation of  ANT leading to permeability transition pore (PTP) opening, loss of  mitochondrial membrane potential, and apoptosis.
|description='''Carboxyatractyloside''', Cat, is a highly selective and potent inhibitor of the [[adenine nucleotide translocator]] (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic (positive) side of the inner membrane and blocks the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the ''c'' conformation of  ANT leading to permeability transition pore (PTP) opening, loss of  mitochondrial membrane potential, and apoptosis.
|info=[[MiPNet03.02]]
|info=[[MiPNet03.02]]
|type=Inhibitor PS
}}
{{MitoPedia methods|type=Inhibitor PS
}}
}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Inhibitor
|mitopedia topic=Inhibitor
|type=Inhibitor PS
}}
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==


'''Cat:  Carboxyatractyloside'''  (carboxyatractyloside potassium salt from ''Xanthium sibiricum'',  C<sub>31</sub>H<sub>44</sub>O<sub>18</sub>S<sub>2</sub>*K<sub>x</sub>);  Sigma C 4992,  2 mg, store at -20 °C; FW = 802.99 (free acid basis); '''Alternative source''': Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from ''Xanthium sibiricum'', C<sub>31</sub>H<sub>43</sub>O<sub>18</sub>S<sub>2</sub>K<sub>3</sub>*3H<sub>2</sub>O), 5 mg, store at -20 °C, FW = 939.1).
::: '''Cat:  Carboxyatractyloside'''  (carboxyatractyloside potassium salt from ''Xanthium sibiricum'',  C<sub>31</sub>H<sub>44</sub>O<sub>18</sub>S<sub>2</sub>*K<sub>x</sub>);  Sigma C 4992,  2 mg, store at -20 °C; FW = 802.99 (free acid basis); '''Alternative source''': Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from ''Xanthium sibiricum'', C<sub>31</sub>H<sub>43</sub>O<sub>18</sub>S<sub>2</sub>K<sub>3</sub>*3H<sub>2</sub>O), 5 mg, store at -20 °C, FW = 939.1).




<span  style="color:#8B008B"> '''Caution:''' Chemicals stored in  the fridge  or freezer should be allowed to reach room temperature  before  opening. '''Very toxic'''!</span>
:::: <span  style="color:#8B008B"> '''Caution:''' Chemicals stored in  the fridge  or freezer should be allowed to reach room temperature  before  opening. '''Very toxic'''!</span>




'''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>0):  
:::: '''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>0):  


::1) Weigh 4.7 mg carboxyatractyloside.
::::# Weigh 4.7 mg carboxyatractyloside.
::2) Dissolve in 1 ml H<sub>2</sub>O.
::::# Dissolve in 1 ml H<sub>2</sub>O.
::3) Divide into 0.2 ml aliquots.
::::# Divide into 0.2 ml aliquots.
::4) Store at -20 °C.
::::# Store at -20 °C.


'''O2k manual titrations'''  >> [[MiPNet09.12 O2k-Titrations]]
::: '''O2k manual titrations'''  » [[MiPNet09.12 O2k-Titrations]]


::* Titration volume: 2 µl using a 10 µl Hamilton syringe (2 ml O2k-chamber).
::::* Titration volume: 2 µl using a 10 µl Hamilton syringe (2 ml O2k-chamber).
::* Final concenteration: 5 µM.
::::* Final concenteration: 5 µM.


'''Comment:''' Cat is considered to be much more specific than [[atractyloside|Atractyloside]] (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.
:::: '''Comment:''' Cat is considered to be much more specific than [[atractyloside|Atractyloside]] (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.




  * [[Talk:Carboxyatractyloside]]
  » [[Talk:Carboxyatractyloside]]

Revision as of 06:05, 14 July 2016


high-resolution terminology - matching measurements at high-resolution


Carboxyatractyloside

Description

Carboxyatractyloside, Cat, is a highly selective and potent inhibitor of the adenine nucleotide translocator (ANT). Cat stabilizes the nucleoside binding site of ANT on the cytoplasmic (positive) side of the inner membrane and blocks the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.

Abbreviation: Cat

Reference: MiPNet03.02


MitoPedia topics: Inhibitor 

Application in HRR

Cat: Carboxyatractyloside (carboxyatractyloside potassium salt from Xanthium sibiricum, C31H44O18S2*Kx); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); Alternative source: Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H43O18S2K3*3H2O), 5 mg, store at -20 °C, FW = 939.1).


Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Very toxic!


Preparation of 5 mM stock solution (dissolved in H20):
  1. Weigh 4.7 mg carboxyatractyloside.
  2. Dissolve in 1 ml H2O.
  3. Divide into 0.2 ml aliquots.
  4. Store at -20 °C.
O2k manual titrations  » MiPNet09.12 O2k-Titrations
  • Titration volume: 2 µl using a 10 µl Hamilton syringe (2 ml O2k-chamber).
  • Final concenteration: 5 µM.
Comment: Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.


» Talk:Carboxyatractyloside