Difference between revisions of "Cannon 2008 Methods Mol Biol"
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|abstract=Brown and white adipose tissues in mammals have a number of similar properties, such as lipid storage and adipokine production, but also distinctive properties. The energy-storing white adipose tissue has few mitochondria and low oxidative capacity. The heat-producing brown adipose tissue has a high density of mitochondria and high oxidative capacity. Mitochondrial function can be investigated in cells and organelles isolated from both brown and white adipose tissues. This chapter describes methods for successful isolation of suitable preparations of adipose tissues and their subsequent use. Questions concerning thermogenic capacity of the tissues, their potential influence on whole body metabolism, and specific properties of the mitochondria and their mode of function may be addressed using these methods. | |abstract=Brown and white adipose tissues in mammals have a number of similar properties, such as lipid storage and adipokine production, but also distinctive properties. The energy-storing white adipose tissue has few mitochondria and low oxidative capacity. The heat-producing brown adipose tissue has a high density of mitochondria and high oxidative capacity. Mitochondrial function can be investigated in cells and organelles isolated from both brown and white adipose tissues. This chapter describes methods for successful isolation of suitable preparations of adipose tissues and their subsequent use. Questions concerning thermogenic capacity of the tissues, their potential influence on whole body metabolism, and specific properties of the mitochondria and their mode of function may be addressed using these methods. | ||
|keywords=Adipose tissue, Heat production, Mitochondria | |keywords=Adipose tissue, Heat production, Mitochondria | ||
|mipnetlab=SE Stockholm Nedergaard J | |||
|discipline=Mitochondrial Physiology, Biomedicine | |discipline=Mitochondrial Physiology, Biomedicine | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |||
|tissues=Endothelial;epithelial;mesothelial cell | |tissues=Endothelial;epithelial;mesothelial cell | ||
|preparations=Isolated mitochondria | |preparations=Isolated mitochondria | ||
| | |couplingstates=LEAK, OXPHOS, ET | ||
| | |pathways=N | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Mitochondrial Physiology, Biomedicine | |discipline=Mitochondrial Physiology, Biomedicine | ||
}} | }} | ||
Latest revision as of 17:10, 9 November 2017
| Cannon B, Nedergaard J (2008) Studies of thermogenesis and mitochondrial function in adipose tissues. Methods Mol Biol 456:109-21. |
Cannon B, Nedergaard J (2008) Methods Mol Biol
Abstract: Brown and white adipose tissues in mammals have a number of similar properties, such as lipid storage and adipokine production, but also distinctive properties. The energy-storing white adipose tissue has few mitochondria and low oxidative capacity. The heat-producing brown adipose tissue has a high density of mitochondria and high oxidative capacity. Mitochondrial function can be investigated in cells and organelles isolated from both brown and white adipose tissues. This chapter describes methods for successful isolation of suitable preparations of adipose tissues and their subsequent use. Questions concerning thermogenic capacity of the tissues, their potential influence on whole body metabolism, and specific properties of the mitochondria and their mode of function may be addressed using these methods. • Keywords: Adipose tissue, Heat production, Mitochondria
• O2k-Network Lab: SE Stockholm Nedergaard J
Labels: MiParea: Respiration
Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N
HRR: Oxygraph-2k
