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Difference between revisions of "Brennan 2006 Biochim Biophys Acta"

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Brennan CM, Breen EP, Porter RK (2006) Cold acclimation and oxygen consumption in the thymus. Biochim. Biophys. Acta 1757: 1463-1468.

Β» PMID: 17022934

Brennan CM, Breen EP, Porter RK (2006) Biochim. Biophys. Acta

Abstract: Mitochondrial uncoupling protein 1 is usually associated with brown adipose tissue but has recently been discovered in rat and mouse thymus. We wished to establish whether there was a thermogenic role for UCP 1 in thymus and thus examined the effect of 5 weeks cold-acclimation on rat thymus tissue abundance, thymocyte oxygen consumption, thymus mitochondrial abundance, uncoupling protein 1 expression and function. We found that thymocytes from cold-acclimated rats had oxygen consumption rates 8 times less than those from rats held at room temperature and that thymocytes from cold-acclimated rats or rats kept at room temperature were noradrenaline insensitive. In addition, we found that thymus tissue or mitochondrial abundance was not increased after cold-acclimation. However uncoupling protein 1 expression per unit mass of mitochondria was increased after cold-acclimation, as determined by immunoblotting (not, vert, similar 1.7-fold) and GDP binding (not, vert, similar 1.5-fold). Consistent with our protein expression data, we also observed an increased, state 4 (not, vert, similar 1.5-fold), GDP-inhibitable (not, vert, similar 1.3-fold) and palmitate activatable (not, vert, similar 1.6-fold) oxygen consumption rates in isolated thymus mitochondria. However, extrapolation of our data showed that cold-acclimation only increased the amount of UCP 1 per gram of thymus tissue not, vert, similar 1.2-fold. Taken together, we conclude that UCP 1 does not have a thermogenic role in thymus. β€’ Keywords: Uncoupling protein-1, Thymus, Thymocyte, Mitochondria, Purine nucleotide, Oxygen consumption

β€’ O2k-Network Lab: IE Dublin Porter RK


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Organism: Mouse, Rat  Tissue;cell: Islet Cell; Pancreas; Thymus"Islet Cell; Pancreas; Thymus" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property., Fat; Adipocytes"Fat; Adipocytes" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 



HRR: Oxygraph-2k