Bioblast alert 2013(02)
- MitoPedia news: The uncoupler CCCP - Carbonyl cyanide m-chloro phenyl hydrazone: In our on-going experiments with various sources of mitochondria and different mt-preparations, we observed that results on respiration and mt-membrane potential were fully comparable using CCCP or FCCP as uncouplers (protonophores) in parallel experiments. Therefore, from now on our lab will replace FCCP by CCCP. Optimum concentrations for the estimation of ETS capacity are slightly higher for CCCP compared to FCCP. Importantly, CCCP is far less expensive than FCCP (protocol development supported by MitoCom).
- Selection of appropriate methodologies: A recently published review by Perry and colleagues provides a critical assessment of currently used methodologies and instruments used in respirometry – excellent reading. Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.
- MitoPedia Glossary - new section: Respiratory control ratios.
Bioblast alert: for information purposes, not intended to be an advertisement. Contributions are welcome.
Power-O2k
Multiple O2k-units are offered as a ‘Power-O2k System’ of high-resolution respirometry for high-throughput with high output. Many MiPNet Laboratories have increased their high-resolution throughput by multiple O2k-units: Power-O2k
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