Bal 2012 PLOS ONE

Bal W, Kurowska E, Maret W (2012) The final frontier of pH and the undiscovered country beyond. PLOS ONE 7(9):e45832.

» https://doi.org/10.1371/journal.pone.0045832

Bal W, Kurowska E, Maret W (2012) PLOS ONE

Abstract: The comparison of volumes of cells and subcellular structures with the pH values reported for them leads to a conflict with the definition of the pH scale. The pH scale is based on the ionic product of water, K_w = [H⁺]×[OH⁻]. We used K_w [in a reversed way] to calculate the number of undissociated H₂O molecules required by this equilibrium constant to yield at least one of its daughter ions, H⁺ or OH⁻ at a given pH. In this way we obtained a formula that relates pH to the minimal volume V_pH required to provide a physical meaning to K_w, (where N_A is Avogadro’s number). For example, at pH 7 (neutral at 25 °C) V_pH = 16.6 aL. Any deviation from neutral pH results in a larger V_pH value. Our results indicate that many subcellular structures, including coated vesicles and lysosomes, are too small to contain free H⁺ ions at equilibrium, thus the definition of pH based on K_w is no longer valid. Larger subcellular structures, such as mitochondria, apparently contain only a few free H⁺ ions. These results indicate that pH fails to describe intracellular conditions, and that water appears to be dissociated too weakly to provide free H⁺ ions as a general source for biochemical reactions. Consequences of this finding are discussed.

• Bioblast editor: Gnaiger E

Template:Cited in Gnaiger 2000 BEC MitoPathways

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Preparation: Isolated mitochondria 

Regulation: pH 

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