Difference between revisions of "BAM15"

Revision as of 14:59, 21 May 2014

high-resolution terminology - matching measurements at high-resolution

BAM15

2,4-dinitrophenol (C₆H₄N₂O₅; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.

Abbreviation: DNP

Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol

MitoPedia topics: Uncoupler

Preparation of 10 mM DNP stock solution (dissolved in H₂O):

Caution: Toxic!

1. Weigh 3.7 mg of DNP (FW = 184.11).

2. Dissolve in 1.2 ml H2O.

3. Neutralize with 1 N KOH.

4. Adjust final volume to 2 ml and divide into 0.2 ml portions.

6. Store at -20 °C.

@@ Line 1: / Line 1: @@
-'''2-fluorophenyl){6-[(2-fluorophenyl)amino](1,2,5-oxadiazolo[3,4-e]pyrazin-5-yl)}amine''' is a protonophore or [[uncoupler]] of oxidative phosphorylation detected in a screen for uncoupling agents exerting less toxicity than commonly used uncouplers and first described by [[Kennwood 2013 Mol MeTAB| Kennwood et al. 2013]].
-In their comparative study using BAM 15 and [[FCCP]] it was shown to increase oxygen flux to a similar extent as the classical uncoupler, to display a much broader range of concentrations inducing maximum respiration, to elicit no formation of H<sub>2</sub>O<sub>2</sub>, to leave cellular membrane potential unaffected, and to ultimately exert less cytotoxicity.
+{{MitoPedia
+|abbr=DNP
+|description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a protonophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]].
+|info=[[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]]
+|type=Uncoupler
+}}
+{{MitoPedia methods|type=Uncoupler
+}}
+{{MitoPedia topics
+|mitopedia topic=Uncoupler
+|type=Uncoupler
+}}
+'''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O):
+<span style="color:#8B008B"> '''Caution:''' Toxic!</span>
+::1. Weigh 3.7 mg of DNP (FW = 184.11).
+::2. Dissolve in 1.2 ml H2O.
+::3. Neutralize with 1 N KOH.
+::4. Adjust final volume to 2 ml and divide into 0.2 ml portions.
+::6. Store at -20 °C.