Difference between revisions of "Azide"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | |||
:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 g·mol<sup>-1</sup>. | |abbr=Azd | ||
:::: 2021-03: | |trivial name=Azide | ||
::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | |complete name=Sodum azide | ||
|chem formula=NaN<sub>3</sub> | |||
|molar mass=65.01 | |||
|vendor=Sigma-Aldrich | |||
|product number=S2002 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=26628-22-8 | |||
|h statements=H300, H310, H330, H373, H410 | |||
|h info=fatal if swallowed, fatal in contact with skin, fatal if inhaled, may cause damage to organs through prolonged or repeated exposure (brain), very toxic to aquatic life with long-lasting effects | |||
}}<!--:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 g·mol<sup>-1</sup>.--> | |||
:::: 2021-03: Preparation instructions were updated to take the volume of the solute into account (see [[Volume_of_the_solute]]). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations. | |||
<!--::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span>--> | |||
Revision as of 11:29, 10 June 2021
- high-resolution terminology - matching measurements at high-resolution
Azide
Description
Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).
Abbreviation: Azd
Reference: MiPNet03.02
Application in HRR
- Azd: Azide (Sodum azide; NaN3), Sigma-Aldrich: S2002, store at RT, CAS: 26628-22-8, M = 65.01 g·mol-1
- Hazard statements: H300, H310, H330, H373, H410; fatal if swallowed, fatal in contact with skin, fatal if inhaled, may cause damage to organs through prolonged or repeated exposure (brain), very toxic to aquatic life with long-lasting effects
- Azd: Azide (Sodum azide; NaN3), Sigma-Aldrich: S2002, store at RT, CAS: 26628-22-8, M = 65.01 g·mol-1
- 2021-03: Preparation instructions were updated to take the volume of the solute into account (see Volume_of_the_solute). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
- Preparation of 4 M storage and stock solution (1 mL, dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 260 mg of sodium azide.
- Dissolve in 900 µL H20.
- Divide into 0.5 mL portions.
- Store at -20 °C.
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): ≥50 µL using a 100 µL Hamilton syringe.
- Final concentration: ≥100 mM.
- Preparation of 2 M stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 400 µL of the 4 M stock solution.
- Add 400 µL H2O.
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): ≥25 µL using a 100 µL Hamilton syringe.
- Final concentration: ≥100 mM.
MitoPedia topics:
Inhibitor
