Difference between revisions of "Appaix 2000 Biochim Biophys Acta"
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{{Publication | {{Publication | ||
|title=Appaix F, Minatchy M-N, Riva-Lavieille C, Olivares J, Antonsson B, Saks VA (2000) Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited. Biochim Biophys Acta 1457: 175- | |title=Appaix F, Minatchy M-N, Riva-Lavieille C, Olivares J, Antonsson B, Saks VA (2000) Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited. Biochim Biophys Acta 1457:175-81. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/10773162 PMID: 10773162] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/10773162 PMID: 10773162] | ||
|authors=Appaix F, Minatchy M-N, Riva-Lavieille C, Olivares J, Antonsson B, Saks VA | |authors=Appaix F, Minatchy M-N, Riva-Lavieille C, Olivares J, Antonsson B, Saks VA | ||
|year=2000 | |year=2000 | ||
|journal=Biochim | |journal=Biochim Biophys Acta | ||
|abstract=This paper recalls the earlier work by Keilin, Margoliash and others at the beginning of the 20th century and shows how their results can be used for the rapid solution of new problems of modern science. It describes a rapid and simple spectrophotometric method for quantitative determination of cytochrome c release from isolated mitochondria or permeabilized cells induced by proapoptotic proteins. For this, the Soret (Ξ³) peak at 414 nm in the spectrum of cytochrome c is used. The results of spectrophotometric assay of cytochrome c release are in accord with those of oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes. | |abstract=This paper recalls the earlier work by Keilin, Margoliash and others at the beginning of the 20th century and shows how their results can be used for the rapid solution of new problems of modern science. It describes a rapid and simple spectrophotometric method for quantitative determination of cytochrome c release from isolated mitochondria or permeabilized cells induced by proapoptotic proteins. For this, the Soret (Ξ³) peak at 414 nm in the spectrum of cytochrome c is used. The results of spectrophotometric assay of cytochrome c release are in accord with those of oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes. | ||
|mipnetlab=FR Grenoble Saks VA | |||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|tissues=Heart | |||
|preparations=Permeabilized tissue, Isolated mitochondria | |||
|enzymes=Complex IV;cytochrome c oxidase | |||
|couplingstates=OXPHOS, ET | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
Latest revision as of 16:48, 9 November 2017
| Appaix F, Minatchy M-N, Riva-Lavieille C, Olivares J, Antonsson B, Saks VA (2000) Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited. Biochim Biophys Acta 1457:175-81. |
Appaix F, Minatchy M-N, Riva-Lavieille C, Olivares J, Antonsson B, Saks VA (2000) Biochim Biophys Acta
Abstract: This paper recalls the earlier work by Keilin, Margoliash and others at the beginning of the 20th century and shows how their results can be used for the rapid solution of new problems of modern science. It describes a rapid and simple spectrophotometric method for quantitative determination of cytochrome c release from isolated mitochondria or permeabilized cells induced by proapoptotic proteins. For this, the Soret (Ξ³) peak at 414 nm in the spectrum of cytochrome c is used. The results of spectrophotometric assay of cytochrome c release are in accord with those of oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes.
β’ O2k-Network Lab: FR Grenoble Saks VA
Labels:
Tissue;cell: Heart Preparation: Permeabilized tissue, Isolated mitochondria Enzyme: Complex IV;cytochrome c oxidase
Coupling state: OXPHOS, ET
HRR: Oxygraph-2k
