Difference between revisions of "Ap5A"
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|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP). | |description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP). | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | :::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28 | ||
:::: '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. | :::: <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
::: '''Preparation of 100 mM stock solution''' (dissolved in H2O): | :::: '''Preparation of 100 mM stock solution''' (dissolved in H2O): | ||
::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O | ::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O | ||
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:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber). | ::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber). | ||
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::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ||
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ||
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{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
}} | |||
Revision as of 10:08, 11 September 2020
- high-resolution terminology - matching measurements at high-resolution
Ap5A
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
Application in HRR
- Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt Sigma D4022, store at -20 °C; molecular weight: 1026.28
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 100 mM stock solution (dissolved in H2O):
- For a 2 mg glass vial: dissolve in 19.49 µL H2O
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 50 µM.
References
Abbreviation: Ap5A
MitoPedia topics:
Inhibitor
