- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Antimycin A is an inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).
Abbreviation: Ama
Reference: MiPNet09.12,
MitoPedia topics: Inhibitor
Applications in HRR
Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 5.4 mg of Antimycin A into a small glass vial (Ama is hygroscopic).
- 2) Add 2 ml ethanol.
- 3) Divide into 0.2 ml portions in glass vials.
- 4) Store at -20 °C.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 2.5 µM.
