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Difference between revisions of "Antimycin A"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=Ama
|abbr=Ama
|description='''Antimycin A''' is an inhibitor of [[Complex III]]. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme ''b''<sub>H</sub> to oxidized Q (Qi site inhibitor).
|description='''Antimycin A''' is an inhibitor of [[Complex III]] (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme ''b''<sub>H</sub> to oxidized Q (Qi site inhibitor).
|info=[[MiPNet09.12]]
|info=[[MiPNet09.12]]
|type=Inhibitor ETS
|type=Inhibitor ETS

Revision as of 07:53, 12 October 2015


high-resolution terminology - matching measurements at high-resolution


Antimycin A

Description

Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).

Abbreviation: Ama

Reference: MiPNet09.12



MitoPedia topics: Inhibitor 

Applications in HRR

Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540

Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):

  1. Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
  2. Add 2 ml ethanol.
  3. Divide into 0.2 ml portions in glass vials.
  4. Store at -20 °C.

O2k manual titrations MiPNet09.12 O2k-Titrations

  • Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
  • Final concentration: 2.5 µM.


Compare

  • Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.