Difference between revisions of "Antimycin A"
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'''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: FW = 540 | '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: FW = 540 | ||
<span style="color:#8B008B"> '''Caution:''' '''Very toxic | <span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
'''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol): | '''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol): | ||
: | :# Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic. | ||
: | :# Add 2 ml ethanol. | ||
: | :# Divide into 0.2 ml portions in glass vials. | ||
: | :# Store at -20 °C. | ||
'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
:* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber). | |||
:* Final concentration: 2.5 µM. | |||
== Compare == | == Compare == | ||
* [[Myxothiazol]] | * [[Myxothiazol]] - this CIII inhibitor induces less ROS production but is more expensive than Ama. | ||
Revision as of 08:51, 12 October 2015
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Antimycin A is an inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).
Abbreviation: Ama
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Applications in HRR
Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
- Add 2 ml ethanol.
- Divide into 0.2 ml portions in glass vials.
- Store at -20 °C.
O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 2.5 µM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
