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Difference between revisions of "Antimycin A"

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'''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol):
'''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol):


::1) weigh 5.5 mg of Antimycin A directly into a 2 mL glass vial    !Difficult to weigh - appears to be hydroscopic!
::1) weigh 5.5 mg of Antimycin A directly into a 2 ml glass vial    !Difficult to weigh - appears to be hydroscopic!
::2) add 2 ml Ethanol
::2) add 2 ml Ethanol
::3) divide into 0.2 ml portions
::3) divide into 0.2 ml portions or store in 2 ml glass vial and use sub samples during experiments (to avoid contamination of stock solution)
::4) store in glass vial (recommendation) at -20 °C
::4) store in glass vials (recommendation) at -20 °C

Revision as of 11:43, 22 November 2010


high-resolution terminology - matching measurements at high-resolution


Antimycin A

Description

Inhibitor of Complex III.

Abbreviation: Ama

Reference: MiPNet09.12,


Labels:




Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Flux Control; Additivity; Threshold; Excess Capacity"Flux Control; Additivity; Threshold; Excess Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 



Application in HRR

Ama: Antimycin A (Antimycin A from Streptomyces sp., Sigma A 8674, 25 g, store at -20 °C): FW = 540)

Caution: Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.


Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 5.5 mg of Antimycin A directly into a 2 ml glass vial !Difficult to weigh - appears to be hydroscopic!
2) add 2 ml Ethanol
3) divide into 0.2 ml portions or store in 2 ml glass vial and use sub samples during experiments (to avoid contamination of stock solution)
4) store in glass vials (recommendation) at -20 °C