Difference between revisions of "Antimycin A"

Revision as of 12:40, 22 November 2010

high-resolution terminology - matching measurements at high-resolution

Antimycin A

Description

Abbreviation: Ama

Labels:

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Flux Control; Additivity; Threshold; Excess Capacity"Flux Control; Additivity; Threshold; Excess Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

Application in HRR

Ama: Antimycin A (Antimycin A from Streptomyces sp., Sigma A 8674, 25 g, store at -20 °C): FW = 540)

Caution: Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 5.5 mg of Antimycin A directly in to 2 mL glass vial !Difficult to weigh - appears to be hydroscopic!

2) add 2 ml Ethanol

3) divide into 0.2 ml portions

4) store in glass vial (recommendation) at -20 °C

@@ Line 25: / Line 25: @@
 ::3) divide into 0.2 ml portions
 ::4) store in glass vial (recommendation) at -20 °C
-  !White precipitate will occur, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature!
-::3) neutralize with 5 N KOH (approx. 465 µl)  !ADP will dissolve after addition of KOH!  (this is '''Solution A''')
-::4) check pH and adjust to 7 if necessary
-::5) adjust final volume to 2 ml and divide into 0.2 ml portions
-::6) store frozen at -80 °C
-'''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
-::* Titration volume: 8-20 µl using a 25 µl syringe
-::* Final conc. in 2 ml O2k-chamber: 2-5 mM