Difference between revisions of "Antimycin A"
From Bioblast
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== Applications in [[HRR]] == | == Applications in [[HRR]] == | ||
'''Ama: Antimycin A''' ( | '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 g, store at -20 °C: FW = 540 | ||
<span style="color:#8B008B"> '''Caution:''' '''Very toxic!!! ''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | <span style="color:#8B008B"> '''Caution:''' '''Very toxic!!! ''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
| Line 24: | Line 24: | ||
::4) Store at -20 °C. | ::4) Store at -20 °C. | ||
'''Oxygraph-2k | '''Oxygraph-2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 1 µl using a 10 µl syringe. | ::* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber). | ||
::* Final | ::* Final concentration: 2.5 µM. | ||
== Compare == | == Compare == | ||
* [[Myxothiazol]] | * [[Myxothiazol]] | ||
Revision as of 15:39, 2 May 2014
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Antimycin A is an inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).
Abbreviation: Ama
Reference: MiPNet09.12,
MitoPedia topics: Inhibitor
Applications in HRR
Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 g, store at -20 °C: FW = 540
Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 5.4 mg of Antimycin A into a small glass vial (Ama is hygroscopic).
- 2) Add 2 ml ethanol.
- 3) Divide into 0.2 ml portions in glass vials.
- 4) Store at -20 °C.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 2.5 µM.
