Difference between revisions of "Antimycin A"
From Bioblast
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::* Titration volume: 1 µl using a 10 µl syringe. | ::* Titration volume: 1 µl using a 10 µl syringe. | ||
::* Final conc. in 2 ml O2k-Chamber: 2.5 µM. | ::* Final conc. in 2 ml O2k-Chamber: 2.5 µM. | ||
== Compare == | |||
* [[Myxothiazol]] | |||
Revision as of 05:18, 1 February 2014
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Antimycin A is an inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).
Abbreviation: Ama
Reference: MiPNet09.12,
MitoPedia topics: Inhibitor
Applications in HRR
Ama: Antimycin A (Antimycin A from Streptomyces sp.) Sigma A 8674, 25 g, store at -20 °C: FW = 540
Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 5.4 mg of Antimycin A into a small glass vial (Ama is hygroscopic).
- 2) Add 2 ml ethanol.
- 3) Divide into 0.2 ml portions in glass vials.
- 4) Store at -20 °C.
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe.
- Final conc. in 2 ml O2k-Chamber: 2.5 µM.
