Difference between revisions of "Amp calibration - DatLab"
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|description='''Amp calibration''' | |description='''Amp calibration''' | ||
'''Site under construction!''' | |||
|info=[[MiPNet19.18D_O2k-Series_G_and_DatLab_6:_Calibration]] | |info=[[MiPNet19.18D_O2k-Series_G_and_DatLab_6:_Calibration]] | ||
}} | }} | ||
| Line 11: | Line 13: | ||
}} | }} | ||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
__TOC__ | |||
== O2k-MultiSensor calibration - general == | |||
Select a MultiSensor plot and press F5 to open the calibration window, or select the channel from the Calibration menu, or press the Amp (or pX) buttons in the O2k-status line (e.g. left double click on pX calib.). | Select a MultiSensor plot and press F5 to open the calibration window, or select the channel from the Calibration menu, or press the Amp (or pX) buttons in the O2k-status line (e.g. left double click on pX calib.). | ||
| Line 21: | Line 24: | ||
Cancel: Press cancel to exit the calibration window without saving any changes. | Cancel: Press cancel to exit the calibration window without saving any changes. | ||
== Amp channel: multiple point calibration - DatLab == | |||
In the left panel the current calibration is shown. The right panel is used for a new calibration. | |||
Select the marks to use for the calibration (1). The column "Conc." will be filled automatically with the "values" associated with each mark, see MiPNet19.19. Alternatively, enter the known concentrations manually (2a) or read concentrations from a previous calibration (same mark names) using Copy from file (2b). The values associated with the mark will be updated if the concentration was changed in the calibration window. | |||
More details: [[MiPNet20.14_AmplexRed_H2O2-production]] | |||
DatLab performs a new linear regression after any field has been edited and displays the sensitivity and intercept (3a). Calibration parameters may be entered manually (3a), or be read from a previous calibration with Copy from file (3b), or be Reset to system default (3c). | |||
Show graph Check the linearity of the calibration. The graph can be copied to clipboard. | |||
Revision as of 08:42, 22 August 2017
- high-resolution terminology - matching measurements at high-resolution
Amp calibration - DatLab
Description
Amp calibration
Site under construction!
Reference: MiPNet19.18D_O2k-Series_G_and_DatLab_6:_Calibration
MitoPedia methods: Fluorometry
MitoPedia O2k and high-resolution respirometry:
DatLab
O2k-MultiSensor calibration - general
Select a MultiSensor plot and press F5 to open the calibration window, or select the channel from the Calibration menu, or press the Amp (or pX) buttons in the O2k-status line (e.g. left double click on pX calib.).
Select a channel and edit Channel label and Unit In the Amp calibration window a drop-down menu allows to select mM, µM or nM as Unit, in the pX calibration window 1, 10-3, 10-6 or 10-9 can be selected.
Calibrate: Apply changes (recalculates Signal and Slope).
Cancel: Press cancel to exit the calibration window without saving any changes.
Amp channel: multiple point calibration - DatLab
In the left panel the current calibration is shown. The right panel is used for a new calibration. Select the marks to use for the calibration (1). The column "Conc." will be filled automatically with the "values" associated with each mark, see MiPNet19.19. Alternatively, enter the known concentrations manually (2a) or read concentrations from a previous calibration (same mark names) using Copy from file (2b). The values associated with the mark will be updated if the concentration was changed in the calibration window.
More details: MiPNet20.14_AmplexRed_H2O2-production
DatLab performs a new linear regression after any field has been edited and displays the sensitivity and intercept (3a). Calibration parameters may be entered manually (3a), or be read from a previous calibration with Copy from file (3b), or be Reset to system default (3c). Show graph Check the linearity of the calibration. The graph can be copied to clipboard.
