Difference between revisions of "ATP"
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'''Preparation of 500 mM ATP stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 500 mM ATP stock solution''' (dissolved in H<sub>2</sub>O): | ||
::1) weigh 614.1 mg of ATP (FW = 614.1 | ::1) weigh 614.1 mg of ATP (FW = 614.1 containing 3.5 mol H<sub>2</sub>O/mol) | ||
::2) add 1.2 ml H<sub>2</sub>O | ::2) add 1.2 ml H<sub>2</sub>O | ||
::3) neutralize with 5 N KOH (approx. 400 µl) - ATP will dissolve after addition of KOH! | ::3) neutralize with 5 N KOH (approx. 400 µl) - ATP will dissolve after addition of KOH! | ||
Revision as of 11:14, 16 May 2012
- high-resolution terminology - matching measurements at high-resolution
ATP
Description
Adenosine 5’-triphosphate disodium salt hydrate (T), C10H14N5O13P3Na2.
Abbreviation: T
Reference: MiPNet03.02; MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Application in HRR
T: ATP (Adenosine 5'-triphosphate disodium salt hydrate, C10H14N5O13P3Na2* (H2O)x); Sigma A 2383, 5 g, store at R.T.): FW = 614.1 containing 3.5 mol H2O/mol (FWanhydrous basis = 551.1). Alternative source: Merck 1191-1GM (Calbiochem, Adenosine 5′-Triphosphate Disodium Salt; FW = 551.2; store at -20 °C).
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 500 mM ATP stock solution (dissolved in H2O):
- 1) weigh 614.1 mg of ATP (FW = 614.1 containing 3.5 mol H2O/mol)
- 2) add 1.2 ml H2O
- 3) neutralize with 5 N KOH (approx. 400 µl) - ATP will dissolve after addition of KOH!
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 2 ml and divide into 0.2 ml portions
- 6) store frozen at -80 °C
