Difference between revisions of "ATP"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=T | |abbr=T | ||
|description=Adenosine 5’-triphosphate (T), C<sub>10</sub>H<sub>14</sub>N<sub>5</sub>O<sub>13</sub>P<sub>3</sub>Na<sub>2</sub>. | |description=Adenosine 5’-triphosphate hydrate(T), C<sub>10</sub>H<sub>14</sub>N<sub>5</sub>O<sub>13</sub>P<sub>3</sub>Na<sub>2</sub>. | ||
|info=[[MiPNet03.02]]; [[MiPNet09.12]] | |info=[[MiPNet03.02]]; [[MiPNet09.12]] | ||
|type=Substrate PS | |type=Substrate PS | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''T: ATP''' (Adenosine 5'-triphosphate disodium salt, C<sub>10</sub>H<sub>14</sub>N<sub>5</sub>O<sub>13</sub>P<sub>3</sub>Na<sub>2</sub>); Sigma A 2383, 5 g, store at | '''T: ATP''' (Adenosine 5'-triphosphate disodium salt hydrate, C<sub>10</sub>H<sub>14</sub>N<sub>5</sub>O<sub>13</sub>P<sub>3</sub>Na<sub>2</sub>* xH<sub>2</sub>O); Sigma A 2383, 5 g, store at R.T.): FW = 551.14. Alternative source: Merck 1191-1GM (Calbiochem; FW = 551.2; store at -20 °C). | ||
<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
'''Preparation of 500 mM ATP stock solution''' (dissolved in H<sub>2</sub>O): | |||
::1) weigh 501.3 mg of ADP (FW = 501.3) = (1 mmol ADP) | |||
::2) add 1.2 ml H<sub>2</sub>O (ADP is not dissolved at this stage. | |||
::3) neutralize with 5 N KOH (approx. 450 µl)! ADP will dissolve after addition of KOH! (this is '''Solution A''') | |||
::4) check pH and adjust to 7 if necessary | |||
::5) adjust final volume to 2 ml and divide into 0.2 ml portions | |||
::6) store frozen at -80 °C | |||
Revision as of 10:19, 16 May 2012
- high-resolution terminology - matching measurements at high-resolution
ATP
Description
Adenosine 5’-triphosphate hydrate(T), C10H14N5O13P3Na2.
Abbreviation: T
Reference: MiPNet03.02; MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Application in HRR
T: ATP (Adenosine 5'-triphosphate disodium salt hydrate, C10H14N5O13P3Na2* xH2O); Sigma A 2383, 5 g, store at R.T.): FW = 551.14. Alternative source: Merck 1191-1GM (Calbiochem; FW = 551.2; store at -20 °C).
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 500 mM ATP stock solution (dissolved in H2O):
- 1) weigh 501.3 mg of ADP (FW = 501.3) = (1 mmol ADP)
- 2) add 1.2 ml H2O (ADP is not dissolved at this stage.
- 3) neutralize with 5 N KOH (approx. 450 µl)! ADP will dissolve after addition of KOH! (this is Solution A)
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 2 ml and divide into 0.2 ml portions
- 6) store frozen at -80 °C
