Watala 2016 Int J Pharm

From Bioblast
Publications in the MiPMap
Watala C, Karolczak K, Kassassir H, Siewiera K, Maczynska K, Pieniazek A, Labieniec-Watala M (2016) How do the full-generation poly(amido)amine (PAMAM) dendrimers activate blood platelets? Platelet membrane zeta potential and other membrane-associated phenomena. Int J Pharm 500:379-89.

Β» PMID: 26772535

Watala C, Karolczak K, Kassassir H, Siewiera K, Maczynska K, Pieniazek A, Labieniec-Watala M (2016) Int J Pharm

Abstract: We explored the hypothesis that zeta potential altered by polycations affects blood platelet activation and reactivity, the phenomena associated with membrane lipid fluidity and platelet mitochondrial bioenergetics. PAMAM dendrimers generation- and dose-dependently enhanced zeta potential of platelets (from -10.7 mV to -4.3 mV). Increased expressions of activation markers, P-selectin and the active complex Ξ±IIbΞ²3, as well as significantly enhanced fibrinogen binding occurred upon the in vitro incubation of blood platelets in the presence of PAMAMs G3 and G4 (resp. 62.1% and 69.4% vs. 1.4% and 2.7% in control for P-selectin, P<0.0001). PAMAM dendrimers increased fluidity of platelet membrane lipid bilayer, while they did not affect platelet mitochondria respiration. Increased platelet activation and their responses to agonists in vitro were statistically associated with the revealed alterations in zeta potential. Our results support the hypothesis that polycation-mediated "neutralized" zeta potential may underlie the activating effects of PAMAMs on blood platelets.

Copyright Β© 2016 Elsevier B.V. All rights reserved. β€’ Keywords: Blood flow, Electric charge, Membrane fluidity, Membrane potential, Mitochondrial respiration, Platelet activation, PBMC

β€’ O2k-Network Lab: PL Lodz Watala C

Labels: MiParea: Respiration 

Organism: Human  Tissue;cell: Blood cells, Platelet  Preparation: Intact cells 

Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2016-10, MitoEAGLE blood cells data 

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