Volt 2016 J Pineal Res
Volt H, GarcΓa JA, Doerrier C, DΓaz-Casado ME, Guerra-Librero A, LΓ³pez LC, Escames G, Tresguerres JA, AcuΓ±a-Castroviejo D (2016) Same molecule but different expression: aging and sepsis trigger NLRP3 inflammasome activation, a target of melatonin. J Pineal Res 60:193-205. |
Volt H, Garcia JA, Doerrier C, Diaz-Casado ME, Guerra-Librero A, Lopez LC, Escames G, Tresguerres JA, Acuna-Castroviejo D (2016) J Pineal Res
Abstract: The connection between the innate immune system, clock genes, and mitochondrial bioenergetics was analyzed during aging and sepsis in mouse heart. Our results suggest that the sole NF-ΞΊB activation does not explain the inflammatory process underlying aging; the former also triggers the NLRP3 inflammasome that enhances caspase-1-dependent maturation of IL-1Ξ². In this way, aged mice enter into a vicious cycle as IL-1Ξ² further activates the NF-ΞΊB/NLRP3 inflammasome link. The origin of NF-ΞΊB activation was related to the age-dependent Bmal1/Clock/RORΞ±/Rev-ErbΞ± loop disruption, which lowers NAD(+) levels, reducing the SIRT1 deacetylase ability to inactivate NF-ΞΊB. Consequently, NF-ΞΊB binding to DNA increases, raising the formation of proinflammatory mediators and inducing mitochondrial impairment. The cycle is then closed with the subsequent NLRP3 inflammasome activation. This paired contribution of the innate immune pathways serves as a catalyst to magnify the response to sepsis in aged compared with young mice. Melatonin administration blunted the septic response, reducing inflammation and oxidative stress, and enhancing mitochondrial function at the levels of nonseptic aged mice, but it did not counteract the age-related inflammation. Together, our results suggest that, although with different strengths, chronoinflammaging constitutes the biochemical substrate of aging and sepsis, and identifies the NLRP3 inflammasome as a new molecular target for melatonin, providing a rationale for its use in NLRP3-dependent diseases. β’ Keywords: NF-ΞΊB, NLRP3 inflammasome, Clock genes, Inflammaging, Melatonin, Mitochondria
β’ O2k-Network Lab: ES Granada Acuna-Castroviejo D
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Pharmacology;toxicology
Pathology: Aging;senescence, Sepsis
Organism: Mouse Tissue;cell: Heart Preparation: Permeabilized tissue Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase
Coupling state: OXPHOS, ET Pathway: N, NS HRR: Oxygraph-2k
2016-02, Melatonin