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From Bioblast

NextGen-O2k Template:MitoFit highlights page name The revolutionary all-in-one instrument to conquer mitochondrial disease


Horizon 2020 Framework Programme Project NextGen-O2k

  • EIC-SMEInst-2018-2020 — SME instrument phase 2, Grant Agreement No. 859770, Oroboros Instruments
  • Budget: € 2.35 Million
  • Duration: 24 months
  • Application: 2019-01-09; Pitch in Brussels: 2019-02-15; Granted: 2019-03-05; Start: 2019-06-01

NextGen-O2k press releases

NextGen-O2k press releases


Top 10 reasons for Oroboros.jpg
Oroboros - as a driving force in mitochondrial physiology - extends the analytical and diagnostic power of high-resolution respirometry by integration of NADH- and Q-redox monitoring in the NextGen-O2k. We aim at establishing the Oroboros quality control management for dissemination to our worldwide O2k-Network laboratories. This will become an effective contribution to address the acute reproducibility crisis of scientific investigation. In the spirit of Open Science and global networking, we will enable data sharing across projects and institutions in an Open Access database on mitochondrial physiology and pathology, to resolve the inflation crisis and ultimately the value-impact crisis of present academic publication. This will support key developments in mitochondrial medicine. In addition, we expand our business to algal biotechnology and ecology with the photobiology module of the NextGen-O2k, widening our focus from medicine to environment and climate.

Open positions

» Oroboros Careers

Form the O2k to the NextGen-O2k

Respiration and photosynthesis
Between 1992 and 1994, Oroboros Instruments released its core technology for high-resolution respirometry (HRR), the Oxygraph-2k or O2k, for real-time monitoring of oxygen consumption of mitochondria, tissues, and living cells. Dissolved oxygen is measured using high-resolution polarographic oxygen sensors. Its main advantages are the unique signal stability and resolution of oxygen. HRR is the core technology present in every single of the devices and it will remain the basis of the NextGen-O2k.
In 2012, the updated version of the O2k was launched, the O2k-FluoRespirometer. The integrated Smart Fluo-Sensors combine fluorescence excitation and detection of defined wavelengths that in combination with fluorophores allow for the measurement of additional mitochondrial parameters, such as membrane potential, H2O2 production, calcium concentration, and ATP production.
The unique software DatLab has been developed for data acquisition and analysis. DatLab allows the researcher to perform calibrations (including automatic barometric and temperature adjustments), background correction, experimental SUIT (substrate-uncoupler-inhibitor-titration) protocols and data analysis.
NextGen-O2k Modules
With the O2k core technology, developed exclusively by Oroboros for high-resolution measurement of oxygen flux, the NextGen-O2k was developed.
The NextGen-O2k is a MultiSensor all-in-one instrument for in-depth studies of (1) the mitochondrial role in human diseases, and (2) mitochondria and chloroplasts ability in regulating algal growth and metabolite production rates.
It integrates 2 new modules: (+1) A Q-Module to measure Q-redox states, and (+2) a PB-Module to study PhotoBiology.
In just one device researchers obtain a snapshot of the mitochondrial/chloroplast metabolic profile, saving on experimental time, reagents and samples, minimizing errors and ensuring reproducible and accurate results. In addition, the instruments offer the highest oxygen sensitivity (5 nM detection limit) with an exceptional resolution (wide oxygen concentration range, between 0-1,000 μM) and robustness (>10 years lifespan), being superior to any other market option.

Oroboros products timeline.jpg


High-resolution respirometry (HRR) and unique combinations of the O2k with

NextGen-O2k modules.jpg


Extend high-resolution respirometry (HRR) to the all-in-one device
  • (+1) Cyclic voltammetry: the Q-redox sensor
(+2) Controlled wavelengths and light intensity: PhotoBiology-Module
  • Save on experimental time, reagents and samples
  • Minimize errors and ensure reproducible and accurate results
  • Highest oxygen sensitivity (5 nM detection limit)
  • Exceptional resolution (wide oxygen concentration range, between 0 - 1,000 μM)
  • Robustness (>10 years lifespan)


Our partners WGT-Elektronik and SHTech have been essential in the development of the NextGen-O2k.
WGT Logo 2012.jpg Shtech.png

Early testers
The influence of KOLs and Early testers/adopters will be used to build awareness of NextGen-O2k among researchers, academic and industrial partners. We have confirmed relevant mitochondrial and algal biotechnology researchers who want to collaborate as early testers:


» Technical developments
» Scientific developments
» Documentation
» Exploitation
» Dissemination
» Project management

NextGen-O2k events

MiPNet24.01 IOC139 Schroecken AT2019-06-17
Schroecken AT, 2019 June 17-22 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139.
MiPNet24.04 IOC140 Amsterdam NL2019-06-23
Amsterdam NL, 2019 Jun 23 Pre-conference Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC140.
OroDM01 Innsbruck AT2019-07-01
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Innsbruck AT, 2019 Jul 01-03 Oroboros Distributor Meeting, OroDM01.
MiPschool Coimbra 20192019-07-08
Coimbra, PT. 2019 Jul 08-11, 12th MiP/MitoEAGLE Training School 2019. Mitochondrial respiratory physiology: Challenges on data sharing, reproducibility, and interpretation.
NextGen-O2k SME2 coaching Orily Pratt part 12019-07-22
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Innsbruck AT, 2019 Jul 22-23 NextGen-O2k SME2 coaching Orily Pratt (part 1), NextGen-O2k SME2.
Mitochondrial Physiology ‐ from Organelle to Organism 2019 Copenhagen DK2019-08-19
Copenhagen DK , 2019 Aug 19-23. Mitochondrial Physiology ‐ from Organelle to Organism
FEBS Workshop Ageing 2019 Innsbruck AT2019-09-09
Innsbruck, AT. 2019 Sep 09-12, FEBS Workshop “Ageing and Regeneration”.
NextGen-O2k SME2 coaching Samia Kappe part 12019-09-12
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Innsbruck AT, 2019 Sep 10-11 NextGen-O2k SME2 coaching Samia Kappe (part 1), NextGen-O2k SME2.
MiPNet24.02 IOC141 Schroecken AT2019-09-23
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Schroecken AT, 2019 Sep 23-28 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC141.
MiPNet24.13 IOC142 Fukuoka JP2019-10-03
ASMRM & J-mit 2019, Fukuoka JP, 2019 Oct 03 Oroboros Lunch Seminar at ASMRM & J-mit 2019 Fukuoka JP - Explore the Mito-World! (IOC142).
ASMRM & J-mit 2019 Fukuoka JP2019-10-03
Fukuoka, JP, 2019 Oct 03-05. Joint ASMRM and J-mit Conference
MiP2019/MitoEAGLE Belgrade RS2019-10-13
Belgrade RS, 13-16 Oct 2019. 14th Conference on Mitochondrial Physiology: Mitochondrial function: changes during life cycle and in noncommunicable diseases - COST MitoEAGLE perspectives and MitoEAGLE WG and MC Meeting.
MiPNet24.15 IOC143 Haryana IN2019-11-15
Haryana IN, 2019 Nov 15. Oroboros O2k-Workshop on high-resolution respirometry: Assessment of mitochondrial function in health and disease IOC143.
Neurocon 2019 Haryana IN2019-11-15
Haryana, IN. 2019 Nov 15-18, Neurocon 2019.
NextGen-O2k SME2 coaching Orily Pratt part 22019-12-03
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Innsbruck AT, 2019 Dec 03-04 NextGen-O2k SME2 coaching Orily Pratt (part 2), NextGen-O2k SME2.
1st Myocardial Function Symposium 2020 Graz AT2020-01-10Graz, AT, 2020 Jan 10-11. 1st Myocardial Function Symposium: “Targets in cardiometabolic disease”.
MiPNet25.03 IOC144 Innsbruck AT2020-01-20
Innsbruck AT, 2020 Jan 20-22. O2k-Coaching Days - Basic IOC144.
MiPNet25.06 IOC145 Innsbruck AT2020-01-27
Innsbruck AT, 2020 Jan 27-29. O2k-Coaching Days - Basic IOC145.
MiPNet25.10 Distributor engagement Tokyo JP2020-02-04
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Tokyo,JP, 2020 Feb 4-7. On-site O2k-Workshop on High-Resolution Respirometry and partner distribution engagement.
MitoEAGLE WG4 Innsbruck AT2020-02-26
Innsbruck AT, 2020 Feb 26-28. WG 4 blood cell workshop and retreat - COST Action MitoEAGLE.
MitoEAGLE WG2 Innsbruck AT2020-03-09
Innsbruck AT, 2020 Mar 9-13. WG 2 on human skeletal muscle fibers retreat - COST Action MitoEAGLE.
12th ÖGMBT Annual Meeting 2020 Virtual Event2020-09-21
Virtual Event, 2020 Sep 21-23. 12th ÖGMBT Annual Meeting - Biomolecules in/for 21st century
Long Night of Research 2020 Virtual Event2020-10-09
Virtual Event, 2020 Oct 09. Oroboros at Long Night of Research, CCB. The diagnostic bioenergetic report – a milestone on the way to mitochondrial fitness and physical well-being.
Analytica China 2020 Shanghai CN2020-11-16Shanghai, CN, 2020 Nov 16-18.Analytica China Exhibition - 10th International Trade Fair for Laboratory Technology, Analysis, Biotechnology and Diagnostics.
2020 PaduaMuscleDays Padua IT2020-11-19Virtual, 2020 Nov 19-21. PaduaMuscleDays - 30 years of translational research
AlgaEurope 2020 Virtual Event2020-12-01
Rome, IT, 2020 Dec 1-5. AlgaEurope 2020.
Career Event - FH Campus 2021 Wien AT2021-01-26Virtual, 2021 Jan 26. Career Event of the FH Campus Wien.
ISAP 2021 Virtual2021-05-14Virtual, 2021 May 14-Aug 13. 7th Conference of the International Society for Applied Phycology - ISAP2021.
24th Kalorimetrietage 2021 Braunschweig DE2021-05-26Braunschweig, DE, 2021 May 26-28. 24th Kalorimetrietage
4th edition Metabolism & Cancer 2021 Virtual2021-05-27Virtual, 2021 May 27-29. 4th edition Metabolism & Cancer.
ESCI 2021 Virtual2021-06-09
Virtual, 2021 Jun 9-11. 55th Annual Scientific Meeting of the European Society for Clinical Investigation, ESCI 2021.
World summit on Diabetes 2021 Virtual2021-06-16Vitual, 2021 Jun 16-17. World Summit on Diabetes: New technologies and practical approaches: Diabetes and Endocrine disorders
4th China Symposium on Nerve Control Metabolism 2021 Hangzhou city CN2021-06-18Hangzhou city, CN, 2021 Jun 18-19. The 4th China Symposium on Neuro-Controlled Metabolism.
MiPNet 26.15 NextGen-O2k Event Photobiology: Algal bioenergetics Innsbruck AT2021-07-15
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Innsbruck AT, 2021 Jul 15-16. NextGen-O2k Event Photobiology: Algal bioenergetics. MiPNet 26.15.
19th Chinese Biophysics congress 2021 Anhui CN2021-07-23Anhui Province , CN, 2021 Jul 23-26. 19th Chinese Biophysics congress.
19th Beijing Conference and Exhibition on Instrumental Analysis 2021 Beijing CN2021-07-27Beijing, CN, 2021 Sep 27-29. 19th Beijing Conference and Exhibition on Instrumental Analysis.
The 4th Academic Symposium of Metabolic Biology Branch 2021 Guangxi CN2021-07-29Guangxi Province , CN, 2021 Jul 29 - Aug 02. The 4th Academic Symposium of Metabolic Biology Branch of Chinese Biophysical Society.
MiPNet 26.16 NextGen-O2k Summit 2021 Virtual2021-10-20
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Virtual, 2021 Oct 20. NextGen-O2k Summit, NextGen-O2k SME2. MiPNet 26.16.
MiPNet27.02 IOC153 Innsbruck AT2022-07-01
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Innsbruck AT, 2022 Jul 01-02. NextGen-O2k All-in-one Demo Workshop IOC153.
MiPNet27.03 IOC154 Innsbruck AT2022-08-01
Innsbruck AT, 2022 Aug 01-05. O2k-Coaching Days - O2k-FluoRespirometer and NextGen-O2k, IOC154.
MiPNet27.05 Schroecken BEC tutorial-Living Communications pmP2022-09-30
Schroecken AT, 2022 Sep 30-Oct 03. BEC tutorial-Living Communications: pmF to pmP — pre IOC155.
MiPNet27.04 IOC155 Schroecken AT2022-10-03
Schroecken AT, 2022 Oct 03-08. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC155.
Virtual OroDM022023
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Virtual Oroboros Distributor Meeting, OroDM02
MiPNet28.05 IOC158 Innsbruck AT2023-02-27
Innsbruck AT, 2023 Feb 27 - Mar 01. O2k-Coaching Days - Basic, IOC158.
MiPNet28.01 IOC160 Schroecken AT2023-06-19
Schroecken AT, 2023 Jun 19-24. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC160.
MiPNet28.02 IOC Schroecken AT2023-10-02
Schroecken AT, 2023 Oct 02-07. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC.

Gentle Science

In addition to O2k-Workshops and on-site training, Oroboros will disseminate the not-for-profit initiatives on Mitochondrial Physiology and Bioenergetics Science Camps, specialized Mitochondrial Physiology Conferences, and Preprint publications:
» Oroboros is a key player in support of the COST Action MitoEAGLE
» MitoGlobal - the world-wide platform for societies and organizations supporting mitochondrial research and medicine
» MitoEAGLE with cooperation by Oroboros launched MitoFit Preprint Archives - the preprint server for mitochondrial physiology and bioenergetics
» A vision on preprints
» Mitochondrial respiratory states and rates : >530 coauthors join forces towards harmonization on nomenclature
» Gentle Science - recognizes the responsibility of the scientific community - for the quality of science, the quality of life in science, and its mission

MitoEAGLE Q-workshop



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