User talk:Laner Verena/My sandbox

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NextGen-O2k


NextGen-O2k         K-Regio MitoFit         O2k-Innovation         Houska-Award 2012         K-Regio MitoCom         Bioblast 2012


User talk:Laner Verena/My sandbox

The revolutionary all-in-one instrument to conquer mitochondrial disease

NextGen-O2k


Horizon 2020 Framework Programme Project NextGen-O2k

  • EIC-SMEInst-2018-2020 — SME instrument phase 2, Grant Agreement No. 859770, Oroboros Instruments
  • Budget: € 2.35 Million
  • Duration: 24 months
  • Application: 2019-01-09; Pitch in Brussels: 2019-02-15; Granted: 2019-03-05; Start: 2019-06-01


Mission

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Oroboros - as a driving force in mitochondrial physiology - extends the analytical and diagnostic power of high-resolution respirometry by integration of NADH- and Q-redox monitoring in the NextGen-O2k. We aim at establishing the Oroboros quality control management for dissemination to our worldwide O2k-Network laboratories. This will become an effective contribution to address the acute reproducibility crisis of scientific investigation. In the spirit of Open Science and global networking, we will enable data sharing across projects and institutions in an Open Access database on mitochondrial physiology and pathology, to resolve the inflation crisis and ultimately the value-impact crisis of present academic publication. This will support key developments in mitochondrial medicine. In addition, we expand our business to algal biotechnology and ecology with the photobiology module of the NextGen-O2k, widening our focus from medicine to environment and climate.


Form the O2k to the NextGen-O2k

Respiration and photosynthesis
Between 1992 and 1994, Oroboros Instruments released its core technology for high-resolution respirometry (HRR), the Oxygraph-2k or O2k, for real-time monitoring of oxygen consumption of mitochondria, tissues, and living cells. Dissolved oxygen is measured using high-resolution polarographic oxygen sensors. Its main advantages are the unique signal stability and resolution of oxygen. HRR is the core technology present in every single of the devices and it will remain the basis of the NextGen-O2k.
In 2012, the updated version of the O2k was launched, the O2k-FluoRespirometer. The integrated Smart Fluo-Sensors combine fluorescence excitation and detection of defined wavelengths that in combination with fluorophores allow for the measurement of additional mitochondrial parameters, such as membrane potential, H2O2 production, calcium concentration, and ATP production.
The unique software DatLab has been developed for data acquisition and analysis. DatLab allows the researcher to perform calibrations (including automatic barometric and temperature adjustments), background correction, experimental SUIT (substrate-uncoupler-inhibitor-titration) protocols and data analysis.


With the O2k core technology, developed exclusively by Oroboros for high-resolution measurement of oxygen flux, the NextGen-O2k was developed.



Oroboros products timeline.jpg
O2k
High-resolution respirometry (HRR) and unique combinations of the O2k with








NextGen-O2k modules.jpg
NextGen-O2k
Extend high-resolution respirometry (HRR) to the all-in-one device
The NextGen-O2k is a MultiSensor all-in-one instrument for in-depth studies of (1) the mitochondrial role in human diseases, and (2) mitochondria and chloroplasts ability in regulating algal growth and metabolite production rates.
  • (+1) Cyclic voltammetry: the Q-redox sensor
(+2) Controlled wavelengths and light intensity: PhotoBiology-Module
  • Save on experimental time, reagents and samples
  • Minimize errors and ensure reproducible and accurate results
  • Highest oxygen sensitivity (5 nM detection limit)
  • Exceptional resolution (wide oxygen concentration range, between 0 - 1,000 μM)
  • Robustness (>10 years lifespan)







Workpackages

WP1
» Technical developments
WP2
» Scientific developments
WP3
» Documentation
WP4
» Exploitation
WP5
» Dissemination
WP6
» Project management

Partners

Our partners WGT-Elektronik and SHTech have been essential in the development of the NextGen-O2k.
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Shtech.png


Early testers
The influence of KOLs and Early testers/adopters will be used to build awareness of NextGen-O2k among researchers, academic and industrial partners. We have confirmed relevant mitochondrial and algal biotechnology researchers who want to collaborate as early testers:


Biomedical research Algal research
Prof. Anthony Moore - University of Sussex, UK Prof. Tom Bibby - University of Southampton, UK
Prof. Massimo Zeviani, Dr. Carlo Viscomi, Prof. Mervyn Singer - University of Cambridge, UK Dr. Tomas Morosinotto - University of Padova, IT
Prof. Pablo Garcia-Roves - University of Barcelona and Bellvitge Biomedical Research Institute (IDIBELL), ES Dr. Yagut Allahverdiyeva - University of Turku, FI
Prof. Christos Chinopoulos - Semmelweis University, HU Prof. Ted Packard - University of Las Palmas de Gran Canaria, ES
Dr. Anthony Molina - University of San Diego, US Prof. Claire Remacle - University of Liège, BE
Dr. Marten Szibor, Prof. Howard Jacobs - University of Helsinki, FI
Dr. Alexander Karabatsiakis - University of Ulm, DE


NextGen-O2k events

 WhenWhere
MiPNet24.01 IOC139 Schroecken AT2019-06-17
O2k-Workshops.png
Schroecken AT, 2019 June 17-22 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139.
MiPNet24.04 IOC140 Amsterdam NL2019-06-23
O2k-Network
Amsterdam NL, 2019 Jun 23 Pre-conference Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC140.
OroDM01 Innsbruck AT2019-07-01
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Innsbruck AT, 2019 Jul 01-03 Oroboros Distributor Meeting, OroDM01.
MiPschool Coimbra 20192019-07-08
COST Action MitoEAGLE
MiPsociety
Coimbra, PT. 2019 Jul 08-11, 12th MiP/MitoEAGLE Training School 2019. Mitochondrial respiratory physiology: Challenges on data sharing, reproducibility, and interpretation.
NextGen-O2k SME2 coaching Orily Pratt part 12019-07-22
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Innsbruck AT, 2019 Jul 22-23 NextGen-O2k SME2 coaching Orily Pratt (part 1), NextGen-O2k SME2.
Mitochondrial Physiology ‐ from Organelle to Organism 2019 Copenhagen DK2019-08-19
COST Action MitoEAGLE
Copenhagen DK , 2019 Aug 19-23. Mitochondrial Physiology ‐ from Organelle to Organism
FEBS Workshop Ageing 2019 Innsbruck AT2019-09-09
FEBS
Innsbruck, AT. 2019 Sep 09-12, FEBS Workshop “Ageing and Regeneration”.
NextGen-O2k SME2 coaching Samia Kappe part 12019-09-12
NextGenO2k logo.jpg
Innsbruck AT, 2019 Sep 10-11 NextGen-O2k SME2 coaching Samia Kappe (part 1), NextGen-O2k SME2.
MiPNet24.02 IOC141 Schroecken AT2019-09-23
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Schroecken AT, 2019 Sep 23-28 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC141.
MiPNet24.13 IOC142 Fukuoka JP2019-10-03
O2k-Network
ASMRM & J-mit 2019, Fukuoka JP, 2019 Oct 03 Oroboros Lunch Seminar at ASMRM & J-mit 2019 Fukuoka JP - Explore the Mito-World! (IOC142).
ASMRM & J-mit 2019 Fukuoka JP2019-10-03
ASMRM LOGO.JPG
Fukuoka, JP, 2019 Oct 03-05. Joint ASMRM and J-mit Conference
MiP2019/MitoEAGLE Belgrade RS2019-10-13
COST Action MitoEAGLE
MiPsociety
Belgrade RS, 13-16 Oct 2019. 14th Conference on Mitochondrial Physiology: Mitochondrial function: changes during life cycle and in noncommunicable diseases - COST MitoEAGLE perspectives and MitoEAGLE WG and MC Meeting.
Neurocon 2019 Haryana IN2019-11-15
Neurocon
Haryana, IN. 2019 Nov 15-18, Neurocon 2019.
MiPNet24.15 IOC143 Haryana IN2019-11-15
O2k-Network
Haryana IN, 2019 Nov 15. Oroboros O2k-Workshop on high-resolution respirometry: Assessment of mitochondrial function in health and disease IOC143.
NextGen-O2k SME2 coaching Orily Pratt part 22019-12-03
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Innsbruck AT, 2019 Dec 03-04 NextGen-O2k SME2 coaching Orily Pratt (part 2), NextGen-O2k SME2.
OroDM02 Schroecken AT2020
NextGenO2k logo.jpg
Schroecken AT, Oroboros Distributor Meeting, OroDM02. Originally scheduled for 2020 Jul 06-07 - Postponing the ORODM02 appears to be unavoidable, more information will follow.
1st Myocardial Function Symposium 2020 Graz AT2020-01-10Graz, AT, 2020 Jan 10-11. 1st Myocardial Function Symposium: “Targets in cardiometabolic disease”.
MiPNet25.10 Distributor engagement Tokyo JP2020-02-04
NextGenO2k logo.jpg
Tokyo,JP, 2020 Feb 4-7. On-site O2k-Workshop on High-Resolution Respirometry and partner distribution engagement.
MitoEAGLE WG4 Innsbruck AT2020-02-26
COST Action MitoEAGLE
Innsbruck AT, 2020 Feb 26-28. WG 4 blood cell workshop and retreat - COST Action MitoEAGLE.
MitoEAGLE WG2 Innsbruck AT2020-03-09
COST Action MitoEAGLE
Innsbruck AT, 2020 Mar 9-13. WG 2 on human skeletal muscle fibers retreat - COST Action MitoEAGLE.
36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ2020-09-10Hradec Kralove, CZ, 2020 Sep 10-12 . 36th annual international congress of Czech Nutrition Society
NextGen-O2k Launch event 2021 Innsbruck AT2021-05-03
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Innsbruck AT, 2021 May 3-8. NextGen-O2k Launch event, NextGen-O2k SME2.


NextGen-O2k dissemination

NextGen-O2k press releases


Gentle Science

In addition to O2k-Workshops and on-site training, Oroboros will disseminate the not-for-profit initiatives on Mitochondrial Physiology and Bioenergetics Science Camps, specialized Mitochondrial Physiology Conferences, and Preprint publications:
» Oroboros is a key player in support of the COST Action MitoEAGLE
» MitoGlobal - the world-wide platform for societies and organizations supporting mitochondrial research and medicine
» MitoEAGLE with cooperation by Oroboros launched MitoFit Preprint Archives - the preprint server for mitochondrial physiology and bioenergetics
» A vision on preprints
» Mitochondrial respiratory states and rates : >530 coauthors join forces towards harmonization on nomenclature
» Gentle Science - recognizes the responsibility of the scientific community - for the quality of science, the quality of life in science, and its mission


MitoEAGLE Q-workshop

MitoFit





Open positions

» Oroboros Careers


Support

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