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Template:SUIT text D096

From Bioblast

The SUIT-041 O2 mt D096 protocol provides a common reference for comparison of respiratory control of mitochondrial preparations such as isolated mitochondria, tissue homogenates and permeabilized cells (already permeabilized when they are added to the chamber) in a wide variety of species, tissues and cell types. SUIT-041 O2 mt D096 is specially designed to give information on F-pathway with titration of acylcarnitine as a FAO substrate to determine the optimal concentration. Moreover, the pathway control in OXPHOS state (F, F(N), FN, FNS pathways) can be evaluated by using this SUIT protocol.