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Template:SUIT text D058

From Bioblast

SUIT-003 O2 ce D058 has been designed to analyze the possible inhibitory effect of the Amplex UltraRed assay on the living cells coupling control state. Usually, the Superoxide dismutase and the Horseradish peroxidase do not show any inhibitory effect over the respiration. However, the Amplex UltraRed seems to inhibit ROUTINE respiration and the ET capacity at high concentrations. For this reason, the inhibitory effect of Amplex UltraRed should be tested with each type of sample used prior to the study of ROS production. SUIT-003 AmR ce D059 should be performed simultaneously as a carrier control titration.