Template:SUIT text D042
From Bioblast
The SUIT-014 O2 pfi D042 protocol is designed for tissue fibers that display a preference for glutamate & malate (GM) over pyruvate & malate (PM) to support NADH-linked respiration. Adding pyruvate in the OXPHOS state enables to check if NADH-linked respiration can be further stimulated to determine N-pathway P capacity. SUIT-014 protocols provide an assessment of the coupling control L- P with NADH linked-substrates (GM) and P- E with N- and S-pathways combined (PGMS). The pathway control in OXPHOS state (N, NS) and ET state (NS, S) is also evaluated, covering the contribution of two pathways which are most important in the mitochondria of many species, tissues and cell types.
