Template:SUIT text D038
From Bioblast
SUIT-003 O2 ce D038 (CCP-ce Crabtree(E)) is designed to study coupling control in living cells and the effect of glucose (or fructose; Crabtree effect) on ET capacity. This protocol allows us to omit oligomycin, therefore the LEAK-respiration. If the aim is to analyze only the Crabtree effect, it is recommended to skip the oligomycin step. If there is an interest in studying coupling control, oligomycin should be added to promote LEAK-respiration. To obtain more information about the Crabtree effect, it is ideal to use in parallel the protocol SUIT-003 O2 ce D037 (glucose addition in ROUTINE). SUIT-003 O2 ce D038 can be extended by the cell viability test and the Complex IV module.
