Template:SUIT text D037
From Bioblast
The SUIT-003 O2 ce D037 (CCP-ce Crabtree(R)) is designed to study coupling control in living cells and the effect of glucose (or fructose; Crabtree effect) on ROUTINE respiration. This protocol allows us to omit oligomycin, therefore the LEAK-respiration. If the aim is to analyze only the Crabtree effect, it is recommended to skip the oligomycin step. If there is an interest in studying coupling control, oligomycin should be added to promote LEAK-respiration. To obtain more information about the Crabtree effect, it is ideal to use in parallel the protocol SUIT-003 O2 ce D038 (glucose addition in ET state). SUIT-003 O2 ce D037 can be extended by the cell viability test and the Complex IV module.
