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Template:SUIT-027 O2 ce-pce D065

From Bioblast
MitoPedia: SUIT

Steps and respiratory states

Ce1;1Dig;1M;2D;3M;4P;5G;6Ama.png

Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1
  • ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
1Dig Dig ce1;1Dig
  • Optimum effective digitonin concentration for complete plasma membrane permeabilization.
Step State Pathway Q-junction Comment - Events (E) and Marks (M)
1M.05 (ML) ce1;1Dig;1M
  • Low concentration of malate, typically low concentration, does not saturate the N-pathway, and will allow us to find the minimum concentration to stimulate the anaplerosis.
2D ( MP) ce1;1Dig;1M;2D
3M MP N CI ce1;1Dig;1M;2D;3M
4P PMP N CI ce1;1Dig;1M;2D;3M;4P
5G PGMP N CI ce1;1Dig;1M;2D;3M;4P;5G
6Ama ROX ce1;1Dig;1M;2D;3M;4P;5G;6Ama
  • Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).


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