Tan 2015 Cell Metab
Tan AS, Baty JW, Dong L, Bezawork-Geleta A, Endaya B, Goodwin J, Bajzikova M, Kovarova J, Peterka M, Yan B, Pesdar EA, Sobol M, Filimonenko A, Stuart S, Vondrusova M, Kluckova K, Sachaphibulkij K, Rohlena J, Hozak P, Truksa J, Eccles D, Haupt LM, Griffiths LR, Neuzil J, Berridge MV (2015) Mitochondrial genome acquisition restores respiratory function and tumorigenic potential of cancer cells without mitochondrial DNA. Cell Metab 21:81-94. |
Β» PMID: 25565207 Open Access Β»
Tan AS, Baty JW, Dong L, Bezawork-Geleta A, Endaya B, Goodwin J, Bajzikova M, Kovarova J, Peterka M, Yan B, Pesdar EA, Sobol M, Filimonenko A, Stuart S, Vondrusova M, Kluckova K, Sachaphibulkij K, Rohlena J, Hozak P, Truksa J, Eccles D, Haupt LM, Griffiths LR, Neuzil J, Berridge MV (2015) Cell Metab
Abstract: We report that tumor cells without mitochondrial DNA (mtDNA) show delayed tumor growth, and that tumor formation is associated with acquisition of mtDNA from host cells. This leads to partial recovery of mitochondrial function in cells derived from primary tumors grown from cells without mtDNA and a shorter lag in tumor growth. Cell lines from circulating tumor cells showed further recovery of mitochondrial respiration and an intermediate lag to tumor growth, while cells from lung metastases exhibited full restoration of respiratory function and no lag in tumor growth. Stepwise assembly of mitochondrial respiratory (super)complexes was correlated with acquisition of respiratory function. Our findings indicate horizontal transfer of mtDNA from host cells in the tumor microenvironment to tumor cells with compromised respiratory function to re-establish respiration and tumor-initiating efficacy. These results suggest pathophysiological processes for overcoming mtDNA damage and support the notion of high plasticity of malignant cells.
β’ O2k-Network Lab: CZ Prague Neuzil J, AU Queensland Neuzil J, AU Queensland Peart J
O2k-brief
MitoFit news 2015#6
- 2015-06-02: Aggression and mitochondrial fitness taken from a friend: acquisition of mtDNA from host cells. Β»MitoFit news
Labels: MiParea: Respiration, mtDNA;mt-genetics, Patients
Pathology: Cancer
Organism: Human, Mouse Tissue;cell: Endothelial;epithelial;mesothelial cell, Genital, Other cell lines Preparation: Intact cells, Permeabilized cells, Isolated mitochondria Enzyme: Supercomplex
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k
O2k-brief