Suski 2013 Abstract MiP2013
| Suski JM, Karkucinska-Wieckowska A, Pronicki M, Wieckowski MR (2013) Oxidative damage of proteins and the status of antioxidant enzymes in autopsy material from the central nervous system of patients with diagnosed or highly probable mitochondrial diseases. Mitochondr Physiol Network 18.08. |
Link:
MiP2013, Book of Abstracts Open Access
Suski JM, Karkucinska-Wieckowska A, Pronicki M, Wieckowski MR (2013)
Event: MiPNet18.08_MiP2013
Mitochondrial diseases occur as a result of mutations in the nuclear or mitochondrial genome. Such impairment is often associated with dysfunctions in proteins which are part of the respiratory chain. First of all, our aim was to study whether the time elapsed from death until the dissection has significant impact on the amount of detected protein damage and thus affects or falsifies the results.
To address this problem, the material (brains) was obtained from mice. After sacrificing, the animals were stored under the same conditions as in the procedures dealing with the remains of patients. Dissection material was collected in a similar time regime, to what is done when obtaining autopsy material from the deceased patients.
The results demonstrated no significant alterations in the profiles of succecive time point analyses in studied proteins levels and damage. This work should allow the future use of autopsy material in the study of oxidative damage, which can help and direct further research toward the diagnosis of mitochondrial diseases, which in the vast majority is accompanied by elevated levels of reactive oxygen species.
Labels: MiParea: mt-Medicine
Stress:Ischemia-reperfusion, Oxidative stress;RONS Organism: Mouse Tissue;cell: Nervous system
MiP2013
Affiliations and author contributions
1 - Department of Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland. 2 - Department of Pathology, The Children’s Memorial Health Institute, Warsaw, Poland.
Email: [email protected]
Supported by a PhD fellowship from the Foundation for Polish Science. This work was supported by the Internal Projects of CMHI 125/2012, Iuventus Plus (UMO-0531/IP1/2011/71) and a grant from the Polish National Science Centre (UMO-2011/01/M/NZ3/02128).
