Stampley 2023 Physiol Rep

Stampley JE, Cho E, Wang H, Theall B, Johannsen NM, Spielmann G, Irving BA (2023) Impact of maximal exercise on immune cell mobilization and bioenergetics. https://doi.org/10.14814/phy2.15753

» Physiol Rep 11:e15753. PMID: 37312242 Open Access

Stampley James E, Cho Eunhan, Wang Haoyan, Theall Bailey, Johannsen Neil M, Spielmann Guillaume, Irving Brian A (2023) Physiol Rep

Abstract: Acute aerobic exercise increases the number and proportions of circulating peripheral blood mononuclear cells (PMBC) and can alter PBMC mitochondrial bioenergetics. In this study, we aimed to examine the impact of a maximal exercise bout on immune cell metabolism in collegiate swimmers. Eleven (7 M/4F) collegiate swimmers completed a maximal exercise test to measure anaerobic power and capacity. Pre- and postexercise PBMCs were isolated to measure the immune cell phenotypes and mitochondrial bioenergetics using flow cytometry and high-resolution respirometry. The maximal exercise bout increased circulating levels of PBMCs, particularly in central memory (KLRG1+/CD57-) and senescent (KLRG1+/CD57+) CD8+ T cells, whether measured as a % of PMBCs or as absolute concentrations (all p < 0.05). At the cellularlevel, the routine oxygen flow (IO₂ [pmol·s^-1 ·10⁶ PBMCs^-1 ]) increased following maximal exercise (p = 0.042); however, there were no effects of exercise on the IO₂ measured under the LEAK, oxidative phosphorylation (OXPHOS), or electron transfer (ET) capacities. There were exercise-induced increases in the tissue-level oxygen flow (IO₂-tissue [pmol·s^-1 ·mL blood^-1 ]) for all respiratory states (all p < 0.01), except for the LEAK state, after accounting for the mobilization of PBMCs. Future subtype-specific studies are needed to characterize further maximal exercise's true impact on immune cell bioenergetics. • Keywords: PBMC, Athletes, Inflammation, Mitochondria, Oxygraph, Respirometry • Bioblast editor: Plangger M • O2k-Network Lab: US LA Baton Rouge Irving BA

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 

Organism: Human  Tissue;cell: Blood cells  Preparation: Permeabilized cells, Intact cells 

Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, NS, ROX  HRR: Oxygraph-2k 

2023-06, PBMCs