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Lasalvia 2018 PLOS ONE
Additional label Labels  + , 2018-04  +
Coupling states LEAK  + , ET  +
Has abstract Blood is a fluid connective tissue of huma
Blood is a fluid connective tissue of human body, where it plays vital functions for the nutrition, defense and well-being of the organism. When circulating in peripheral districts, it is exposed to some physical stresses coming from outside the human body, as electromagnetic fields (EMFs) which can cross the skin. Such fields may interact with biomolecules possibly inducing non thermal-mediated biological effects at the cellular level. In this study, the occurrence of biochemical/biological modifications in human peripheral blood lympho-monocytes exposed in a reverberation chamber for times ranging from 1 to 20 h to EMFs at 1.8 GHz frequency and 200 V/m electric field strength was investigated. Morphological analysis of adherent cells unveiled, in some of these, appearance of an enlarged and deformed shape after EMFs exposure. Raman spectra of the nuclear compartment of cells exposed to EMFs revealed the onset of biochemical modifications, mainly consisting in the reduction of the DNA backbone-linked vibrational modes. Respirometric measurements of mitochondrial activity in intact lympho-monocytes resulted in increase of the resting oxygen consumption rate after 20 h of exposure, which was coupled to a significant increase of the FoF1-ATP synthase-related oxygen consumption. Notably, at lower time-intervals of EMFs exposure (i.e. 5 and 12 h) a large increase of the proton leak-related respiration was observed which, however, recovered at control levels after 20 h exposure. Confocal microscopy analysis of the mitochondrial membrane potential supported the respiratory activities whereas no significant variations in the mitochondrial mass/morphology was observed in EMFs-exposed lympho-monocytes. Finally, altered redox homeostasis was shown in EMFs-exposed lympho-monocytes, which progressed differently in nucleated cellular subsets. These results suggest the occurrence of adaptive mechanisms put in action, likely via redox signaling, to compensate for early impairments of the oxidative phosphorylation system caused by exposure to EMFs. Overall the data presented warn for health safety of people involved in long-term exposure to electromagnetic fields, although further studies are required to pinpoint the leukocyte cellular subset(s) selectively targeted by the EMFs action and the mechanisms by which it is achieved.
nd the mechanisms by which it is achieved.  +
Has editor [[Kandolf G]]  +
Has info [ PMID: 29462174 Open Access]  +
Has title Lasalvia M, Scrima R, Perna G, Piccoli C,
Lasalvia M, Scrima R, Perna G, Piccoli C, Capitanio N, Biagi PF, Schiavulli L, Ligonzo T, Centra M, Casamassima G, Ermini A, Capozzi V (2018) Exposure to 1.8 GHz electromagnetic fields affects morphology, DNA-related Raman spectra and mitochondrial functions in human lympho-monocytes. PLOS ONE 13:e0192894.
an lympho-monocytes. PLOS ONE 13:e0192894.  +
Instrument and method Oxygraph-2k  +
Mammal and model Human  +
MiP area Respiration  +
Preparation Intact cells  +
Tissue and cell Lymphocyte  +
Was published by MiPNetLab IT Foggia Capitanio N +
Was published in journal PLOS ONE +
Was published in year 2018  +
Was written by Lasalvia M + , Scrima R + , Perna G + , Piccoli C + , Capitanio N + , Biagi PF + , Schiavulli L + , Ligonzo T + , Centra M + , Casamassima G + , Ermini A + , Capozzi V +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
15:02:25, 11 April 2018  +
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