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Burney 2018 Thesis
Additional label 2018-08  + , Amplex UltraRed  +
Coupling states LEAK  + , ROUTINE  + , OXPHOS  + , ET  +
Has abstract The prevalence of obesity is increasing wo
The prevalence of obesity is increasing worldwide, causing a subsequent increase in insulin resistance (IR), metabolic disorders, and cardiovascular diseases. Although these conditions significantly impact both the individual and society, their etiologies have not been discovered, inhibiting potential prevention and treatment. Alterations to mitochondrial metabolism are implicated in the development of IR and progression to Type 2 Diabetes (T2D), raising the need for an investigation of mitochondrial metabolism in skeletal muscle. Understanding metabolism on an individual substrate basis within a model system enables identification of each nutrient’s contribution to respiration and potential mitochondrial dysfunction. As such, the purpose of this investigation was to characterize mitochondrial metabolism and determine mitochondrial respiratory control in L6 rat myoblasts. High-resolution respirometry and three Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocols were used to examine respiration. Mitochondrial capacity was not limited by the electron transfer system, although the presence of multiple substrates potentially flooded the Q-junction, resulting in lower respiratory control and indicating the presence of a potential limiting factor. In sum, L6 myoblasts exhibit no overt defects in mitochondrial metabolism, but may have a propensity for substrate overload, thereby limiting respiration.
te overload, thereby limiting respiration.  +
Has editor [[Plangger M]]  +
Has info [ Open Access]  +
Has publicationkeywords Mitochondria  + , Oxidative Phosphorylation  + , Electron transfer  + , L6 rat myoblasts  +
Has title Burney E (2018) Characterization of mitochondrial metabolism in L6 rat myoblasts. Bachelor's Thesis p50.  +
Instrument and method Oxygraph-2k  + , O2k-Fluorometer  +
Mammal and model Rat  +
MiP area Respiration  +
Pathways F  + , N  + , S  + , Gp  + , NS  + , Other combinations  + , ROX  +
Preparation Permeabilized cells  + , Intact cells  +
Tissue and cell Other cell lines  +
Was published in journal Bachelor's Thesis +
Was published in year 2018  +
Was written by Burney E +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
09:03:53, 30 August 2018  +
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