Sarti 2013 Int J Mol Sci

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Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M (2013) New evidence for cross talk between melatonin and mitochondria mediated by a circadian-compatible interaction with nitric oxide. Int J Mol Sci 14:11259-76.

» PMID: 23759982 Open Access

Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M (2013) Int J Mol Sci

Abstract: Extending our previous observations, we have shown on HaCat cells that melatonin, at ~10-9 M concentration, transiently raises not only the expression of the neuronal nitric oxide synthase (nNOS) mRNA, but also the nNOS protein synthesis and the nitric oxide oxidation products, nitrite and nitrate. Interestingly, from the cell bioenergetic point of view, the activated NO-related chemistry induces a mild decrease of the oxidative phosphorylation (OXPHOS) efficiency, paralleled by a depression of the mitochondrial membrane potential. The OXPHOS depression is apparently balanced by glycolysis. The mitochondrial effects described have been detected only at nanomolar concentration of melatonin and within a time window of a few hours' incubation; both findings compatible with the melatonin circadian cycle. Keywords: Nitric oxide; Cell bioenergetics; Respiratory chain; Circadian rhythm; Cell culture; Warburg effect; Reactive oxygen; Nitrogen species

O2k-Network Lab: IT Rome Sarti P


Labels: MiParea: Respiration, Pharmacology;toxicology 


Organism: Human  Tissue;cell: Other cell lines 


Regulation: mt-Membrane potential  Coupling state: OXPHOS 

HRR: Oxygraph-2k 

Melatonin