Sahl 2018 Anal Biochem
|Sahl RE, Morville T, Kraunsøe R, Dela F, Helge JW, Larsen S (2018) Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies. Anal Biochem 556:119-24.|
Abstract: Skeletal muscle is a heterogeneous tissue and it is essential to know the methodological variation and reliability when measuring aspects of muscle function. We assessed the methodological and biological variation when measuring mitochondrial respiratory capacity (MRC), citrate synthase (CS) activity and myosin heavy chain (MHC) composition in muscle biopsies from nine healthy male participants, and in addition we assessed variation in MRC in isolated mitochondria and yeast suspension. We analyzed MRC, CS activity and MHC composition in duplicates (intra-biopsy variation) to quantify the methodological variation, as well as the biological variation from multiple muscle biopsies (inter-biopsy variation) obtained at different sites of the same muscle. Three muscle biopsies (B1, B2 and B3) were obtained from each subject in m. vastus lateralis. Two of the biopsies were from the same leg and one from the other leg.
For MRC, intra-biopsy coefficient of variation (CV) was 8.4 % and inter-biopsy CV was 13.3 %. For MHC type I, IIa and IIx intra-biopsy CV was 8.3, 6.0 and 22.3 %, respectively. Inter-biopsy CV for these MHC types were 21.5, 15.4 and 42.0 %, respectively. For CS activity intra-biopsy CV was 0.6 % and inter-biopsy CV was 15.3 %. No differences between B1, B2 and B3 were detected for MRC, CS activity or MHC composition.
• Keywords: Coefficient of variance, Human skeletal muscle, Mitochondrial respiratory capacity, Myosin heavy chain, SDS-PAGE • Bioblast editor: Plangger M, Kandolf G • O2k-Network Lab: DK Copenhagen Dela F, DK Copenhagen Larsen S
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Organism: Human, Mouse, Saccharomyces cerevisiae Tissue;cell: Skeletal muscle Preparation: Intact cells, Permeabilized cells, Isolated mitochondria
Coupling state: OXPHOS, ET Pathway: N, NS HRR: Oxygraph-2k