Risiglione 2022 Life (Basel)
| Risiglione P, Cubisino SAM, Lipari CLR, De Pinto V, Messina A, Magrì A (2022) α-Synuclein A53T promotes mitochondrial proton gradient dissipation and depletion of the organelle respiratory reserve in a neuroblastoma cell line. https://doi.org/10.3390/life12060894 |
Β» Life (Basel) 12:894. PMID: 35743925 Open Access
Risiglione Pierpaolo, Cubisino Salvatore Antonio Maria, Lipari Cristiana Lucia Rita, De Pinto Vito, Messina Angela, Magri Andrea (2022) Life (Basel)
Abstract: Ξ±-synuclein (Ξ±Syn) is a small neuronal protein whose accumulation correlates with Parkinson's disease. Ξ±Syn A53T mutant impairs mitochondrial functions by affecting substrate import within the organelle, activity of complex I and the maximal respiratory capacity. However, the precise mechanism initiating the bioenergetic dysfunction is not clearly understood yet. By overexpressing Ξ±Syn A53T in SH-SY5Y cells, we investigated the specific changes in the mitochondrial respiratory profile using High-Resolution Respirometry. We found that Ξ±Syn A53T increases dissipative fluxes across the intermembrane mitochondrial space: this does not compromise the oxygen flows devoted to ATP production while it reduces the bioenergetic excess capacity of mitochondria, providing a possible explanation of the increased cell susceptibility observed in the presence of further stress stimuli. β’ Keywords: Parkinsonβs disease, High-resolution respirometry, Mitochondrial dysfunction, Ξ±Syn β’ Bioblast editor: Plangger M β’ O2k-Network Lab: IT Catania Messina A
Labels: MiParea: Respiration, Genetic knockout;overexpression
Pathology: Parkinson's
Organism: Human Tissue;cell: Neuroblastoma Preparation: Permeabilized cells, Intact cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
2022-11
