Presby 2021 Diabetes

Presby DM, Rudolph MC, Sherk VD, Jackman MR, Foright RM, Jones KL, Houck JA, Johnson GC, Higgins JA, Neufer PD, Eckel RH, MacLean PS (2021) Lipoprotein lipase overexpression in skeletal muscle attenuates weight regain by potentiating energy expenditure. https://doi.org/10.2337/db20-0763

» Diabetes 70:867-77. PMID: 33536195 Open Access

Presby David M, Rudolph Michael C, Sherk Vanessa D, Jackman Matthew R, Foright Rebecca M, Jones Kenneth L, Houck Julie A, Johnson Ginger C, Higgins Janine A, Neufer P Darrell, Eckel Robert H, MacLean Paul S (2021) Diabetes

Abstract: Moderate weight loss improves numerous risk factors for cardiometabolic disease; however, long-term weight loss maintenance (WLM) is often thwarted by metabolic adaptations that suppress energy expenditure and facilitate weight regain. Skeletal muscle has a prominent role in energy homeostasis; therefore, we investigated the effect of WLM and weight regain on skeletal muscle in rodents. In skeletal muscle of obesity-prone rats, WLM reduced fat oxidative capacity and downregulated genes involved in fat metabolism. Interestingly, even after weight was regained, genes involved in fat metabolism were also reduced. We then subjected mice with skeletal muscle lipoprotein lipase overexpression (mCK-hLPL), which augments fat metabolism, to WLM and weight regain and found that mCK-hLPL attenuates weight regain by potentiating energy expenditure. Irrespective of genotype, weight regain suppressed dietary fat oxidation and downregulated genes involved in fat metabolism in skeletal muscle. However, mCK-hLPL mice oxidized more fat throughout weight regain and had greater expression of genes involved in fat metabolism and lower expression of genes involved in carbohydrate metabolism during WLM and regain. In summary, these results suggest that skeletal muscle fat oxidation is reduced during WLM and regain, and therapies that improve skeletal muscle fat metabolism may attenuate rapid weight regain.

• Bioblast editor: Plangger M • O2k-Network Lab: US CO Aurora Jackman MR, US NC Greenville Neufer PD

Labels: MiParea: Respiration 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

HRR: Oxygraph-2k 

2023-08