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Pichaud 2013 J Exp Biol

From Bioblast
Publications in the MiPMap
Pichaud N, Garratt M, Ballard JWO, Brooks RC (2013) Physiological adaptations to reproduction II. Mitochondrial adjustments in livers of lactating mice. J Exp Biol 216:2889-95.

ยป PMID: 23619407 Open Access

Pichaud N, Garratt M, Ballard JWO, Brooks RC (2013) J Exp Biol

Abstract: Reproduction imposes significant costs and is characterized by an increased energy demand. As a consequence, individuals adjust their cellular structure and function in response to this physiological constraint. Because mitochondria are central to energy production, changes in their functional properties are likely to occur during reproduction. Such changes could cause adjustments in reactive oxygen species (ROS) production and consequently in oxidative stress levels. In this study, we investigated several mechanisms involved in energy production, including mitochondrial respiration at different steps of the electron transport system (ET-pathway) and related the results to citrate synthase activity in the liver of non-reproductive and reproductive (two and eight pups) female house mice at peak lactation. Whereas we did not find differences between females having different litter sizes, liver mitochondria of reproductive females showed lower ET activity and an increase in mitochondrial density when compared with the non-reproductive females. Although it is possible that these changes were due to combined processes involved in reproduction and not to the relative investment in lactation, we propose that the mitochondrial adjustment in liver might help to spare substrates and therefore energy for milk production in the mammary gland. Moreover, our results suggest that these changes lead to an increase in ROS production that subsequently upregulates antioxidant defence activity and decreases oxidative stress. โ€ข Keywords: Citrate synthase, Liver, Metabolism, Mitochondrial respiration, Reproduction

โ€ข O2k-Network Lab: AU Sydney Ballard JW, CA Rimouski Blier PU, CA Moncton Pichaud N

Labels: MiParea: Respiration, mt-Biogenesis;mt-density 

Organism: Mouse  Tissue;cell: Liver  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k