Pichaud 2013 Abstract MiP2013
|Pichaud N, Blier PU(2013) Importance of mitochondrial haplotypes in the expression of metabolic phenotypes under different conditions. Mitochondr Physiol Network 18.08.|
Differential expression of genes mediated by environmental parameters has the potential to influence the organismal phenotype. Considering the central importance of mitochondria in physiological processes such as senescence and life history traits, we hypothesize that expression of mitochondrial genes, under different environmental conditions, should be under strong evolutionary constraints. Integrity of mitochondrial functions requires a synergistic interaction between the mitochondrial and nuclear genomes with proteins produced from mtDNA genes interacting with proteins imported from nuclear encoded genes to produce a functional mitochondrial electron transfer-pathway (ET-pathway). Using permeabilized fibers of Drosophila expressing different mtDNA haplotypes in a homogenous nuclear background, we investigated the effect of several conditions such as aging, temperature and diet on mitochondrial functions. We showed that different set of conditions may trigger the expression of a particular phenotype caused by mtDNA divergences. This is of paramount importance to understand the influence of the environment on mitochondrial evolution in a wide variety of species including humans and has the potential to provide a cohesive picture of the underlying mechanisms of co-evolved ET-pathway Complexes.
• O2k-Network Lab: CA Rimouski Blier PU
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics, nDNA;cell genetics, Exercise physiology;nutrition;life style Pathology: Aging;senescence Stress:Oxidative stress;RONS Organism: Drosophila, Hexapods Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue Enzyme: Complex IV;cytochrome c oxidase, Marker enzyme, TCA cycle and matrix dehydrogenases Regulation: ADP, Flux control, Inhibitor, Oxygen kinetics, Redox state, Substrate, Temperature, Uncoupler Coupling state: LEAK, OXPHOS, ET Pathway: N, S, Gp, CIV, NS, ROX HRR: Oxygraph-2k
Laboratoire de Biologie Intégrative, Université du Québec à Rimouski, Canada. - Email: firstname.lastname@example.org