Orynbayeva 2015 Nanomedicine (Lond)

From Bioblast
Publications in the MiPMap
Orynbayeva Z, Sensenig R, Polyak B (2015) Metabolic and structural integrity of magnetic nanoparticle-loaded primary endothelial cells for targeted cell therapy. Nanomedicine (Lond) 10:1555-68.

Β» PMID: 26008193

Orynbayeva Zulfiya, Sensenig R, Polyak B (2015) Nanomedicine (Lond)

Abstract: Aim: To successfully translate magnetically mediated cell targeting from bench to bedside, there is a need to systematically assess the potential adverse effects of magnetic nanoparticles (MNPs) interacting with β€˜therapeutic’ cells. Here, we examined in detail the effects of internalized polymeric MNPs on primary rat endothelial cells’ structural intactness, metabolic integrity and proliferation potential.

Materials & methods: The intactness of cytoskeleton and organelles was studied by fluorescent confocal microscopy, flow cytometry and high-resolution respirometry. Results: MNPloaded primary endothelial cells preserve intact cytoskeleton and organelles, maintain normal rate of proliferation, calcium signaling and mitochondria energy metabolism.

Conclusion: This study provides supportive evidence that MNPs at doses necessary for targeting did not induce significant adverse effects on structural integrity and functionality of primary endothelial cells – potential cell therapy vectors. β€’ Keywords: Ca2+ signaling, Cytoskeleton, Magnetic nanoparticles, Mitochondria membrane potential, Mitochondria oxidative phosphorylation, Primary endothelial cells

β€’ O2k-Network Lab: US PA Philadelphia Orynbayeva Z

Labels: MiParea: Respiration, mt-Membrane 

Organism: Rat  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Intact cells 

Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

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