Oliveira 2015 Biotechniques
|Oliveira AF, Cunha DA, Ladriere L, Igoillo-Esteve M, Bugliani M, Marchetti P, Cnop M (2015) In vitro use of free fatty acids bound to albumin: A comparison of protocols. Biotechniques 58:228-33.|
Abstract: Long-chain free fatty acids (FFAs) are important metabolic substrates for energy production and lipid synthesis that are also involved in signaling processes (1,2). Two of the most common fatty acids in humans are the long-chain saturated FFA palmitate (C16:0) and the monounsat - urated oleate (C18:1). Western diets rich in fatty acids are associated with increased levels of plasma cholesterol, hepatic steatosis, and a greater risk of cardiovascular disease (3,4). High levels of circulating FFAs, in particular of saturated FFAs, are implicated in insulin resistance and pancreatic b-cell dysfunction, and are predictive of diabetes development (5–8). In vitro exposure to high levels of FFAs leads to lipotoxicity, causing cellular dysfunction and death (5,9).
Labels: MiParea: Exercise physiology;nutrition;life style
Stress:Cell death Organism: Human Tissue;cell: Islet cell;pancreas;thymus