Oemer 2018 Proc Nat Acad Sci U S A
|Oemer G, Lackner L, Muigg K, Krumschnabel G, Watschinger K, Sailer S, Lindner H, Gnaiger E, Wortmann SB, Werner ER, Zschocke J, Keller MA (2018) The molecular structural diversity of mitochondrial cardiolipins. Proc Nat Acad Sci U S A 115:4158-63.|
Abstract: Current strategies used to quantitatively describe the biological diversity of lipids by mass spectrometry are often limited in assessing the exact structural variability of individual molecular species in detail. A major challenge is represented by the extensive isobaric overlap present among lipids, hampering their accurate identification. This is especially true for cardiolipins, a mitochondria-specific class of phospholipids, which are functionally involved in many cellular functions, including energy metabolism, cristae structure, and apoptosis. Substituted with four fatty acyl side chains, cardiolipins offer a particularly high potential to achieve complex mixtures of molecular species. Here we demonstrate, how systematically generated high performance liquid chromatography - tandem mass spectral data can be utilized in a mathematical structural modeling approach, in order to comprehensively analyze and characterize the molecular diversity of mitochondrial cardiolipin compositions in a broad set of biological samples.
Featured on the newspage of the Medical University Innsbruck: Tiefer Blick in die Mitochondrien erweitert Verständnis über Barth-Syndrom
Labels: MiParea: Respiration, mt-Membrane Pathology: Cardiovascular
Organism: Human Tissue;cell: HeLa Preparation: Intact cells, Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: F, N, S, Gp, CIV, NS, ROX HRR: Oxygraph-2k