Michalak S

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MiPsociety
COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


 

Michalak S


MitoPedia topics: EAGLE 

COST: Member


COST WG4: WG4 COST Mentor: Mentor

MC Member - Management Committee MitoEAGLE
Name Michalak Slawomir, Dr
Institution
Michalak S.jpg
Department of Neurochemistry and Neuropathology,

Poznan University of Medical Sciences, PL

Address Przybyszewskiego Str. 49, 60-355
City Poznan
State/Province
Country Poland
Email swami@ump.edu.pl
Weblink
O2k-Network Lab PL Poznan Michalak S


Labels: Field of research: Basic, Clinical 



Publications

 PublishedReference
Gnaiger 2019 MitoFit Preprint Arch2019Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v5.
MitoEAGLE blood cells 12018MitoEAGLE blood cells group (2018) An interlaboratory guide through procedures for mitochondrial respiratory studies with intact and permeabilized peripheral blood mononuclear cells and platelets. - Updated: 2018-02-23
Michalak 2017 Oxid Med Cell Longev2017Michalak S, Florczak-Wyspiańska J, Rybacka-Mossakowska J, Ambrosius W, Osztynowicz K, Baszczuk A, Kozubski W, Wysocka E (2017) Mitochondrial respiration in intact peripheral blood mononuclear cells and sirtuin 3 activity in patients with movement disorders. Oxid Med Cell Longev 2017:9703574.

Abstracts

 PublishedReference
Calabria 2019 MiPschool Coimbra2019
Elisa Calabria
Investigating mitochondrial function in blood cells by high resolution respirometry: contribution to an interlaboratory study. Mitoeagle WG4.
Michalak 2018 MiP20182018
Michalak Slawomir
No abstract.
Michalak 2017 MiP20172017
MiPsociety
The effect of anticoagulants on mitochondrial respiration in intact peripheral blood mononuclear cells.
Rybacka-Mossakowska 2017 MiP20172017
MiPsociety
Mitochondrial respiration in intact peripheral blood mononuclear cells in multiple sclerosis patients.
Michalak 2017 Abstract MITOEAGLE Barcelona2017
COST Action MITOEAGLE
Mitochonrial respiration in intact peripheral blood mononuclear cells in patients with extrapyramidal syndromes.


MitoEAGLE Short-Term Scientific Mission

Work plan summary
1. Aim and motivation. Blood cells, like peripheral mononuclear cells (PBMCs) and platelets, can be obtained with minimal invasive procedures and used for analyzes of mitochondrial respiration for diagnostic and research purposes. Up to now I have analyzed by means of ELISA technique the expression of mitochondrial proteins in PBMCs from movement disorders and cancer patients. With the use of high-resolution respirometry I have analyzed mitochondrial respiration in PBMCs in movement disorders and cancer patients. The results were already published. Moreover, I have performed methodological study on the effects of anticoagulants on mitochondrial respiration in PBMCs. The results of the studies in movement disorders patients and on anticoagulant effects were my contributions to the Working Group 4 in COST Action MITOEAGLE. Currently, I do have the opportunity to perform analyzes of Ca+2 ions and mitochondrial membrane potential basing on tetraphenylphosphonium (TPP+) or TPMP+ evaluations using potentiometric ion-selective electrodes (ISE) and of reactive oxigen species using the system for high-resolution respirometry (HRR). However, ISE and HRR techniques require specific skills I would like to acquire during STSM I apply to and it is the aim of this STMS.
2. Proposed contributions to the scientific objectives of the Action I will contribute the results of the studies on calcium flow, mitochondrial membrane potential and production of reactive oxigen species in PBMCs in physiological and pathological conditions to the repository of Working Group 4 in COST Action MITOEAGLE. Moreover, I will continue the studies on methodology and protocols for mitochondrial analyzes in PBMCs using new techniques I will learn during STSM.
3. Techniques The techniques I will learn during STSM include potentiometric analyses using ion-selective electrodes (ISE) and high-resolution respirometry by means of MultiSensor applications. My training during STSM will fully contribute to the scientific objectives of MitoEAGLE according to the Memorandum of Understanding.


MitoEAGLE feedback

  • I would like to participate in WG4 - MitoEAGLE data repository for blood cells and clutured cells. We are already working with cryopreserved PBMC from lung cancer, breast cancer and colon cancer patients. Thus, I would like to participate in WG4 tasks. - Slawomir Michalak (2016).


Participated at

Visiting scientist in the Oroboros MitoFit Laboratory

O2k-Network

Slawomir Michalak : Visiting scientist at the Oroboros MitoFit Laboratory

  • January 23 to February 03 2018